# US 12,110,556 B2 · Kit for assessing the risk of complications in patients with systemic inflammatory response syndrome (SIRS)

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- Number: US 12,110,556 B2
- Document ID: US12110556B2
- Status: Granted
- Kind: B2
- Application number: 17/729,115
- Filed: 2022-04-26
- Published: 2022-08-18
- Granted: 2024-10-08
- Earliest claimed: 2015-12-01
- Assignees: Biomerieux; Hospices Civils De Lyon; Universite Claude Bernard
- Applicants: Biomerieux; Hospices Civils De Lyon; Universite Claude Bernard Lyon 1
- Inventors: Elisabeth Cerrato; Benjamin Delwarde; Guillaume Monneret; Estelle Peronnet; Julien Textoris; Fabienne Venet
- Web page: https://patentgrep.com/patent/US12110556B2

## CPC

- C12Q 1/6883: for diseases caused by alterations of genetic material
- C12Q 1/6806: Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence)
- C12Q 1/686: Polymerase chain reaction [PCR]
- C12Q 2600/112: Disease subtyping, staging or classification
- C12Q 2600/118: Prognosis of disease development
- C12Q 2600/158: Expression markers
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

A kit for in vitro measurement of a at least one IL7R gene transcript in a blood sample, including specific reagents for measuring the transcript, and a control sample calibrated to contain the IL7R gene transcript corresponding to the mean quantity measured in a pool of reference blood samples from human patients in a state of septic shock when reference blood samples are taken, or who were in a state of septic shock within 72 h after taking the reference blood samples, and who were known to have survived, and/or a calibrated to contain the quantity of an IL7R gene transcript corresponding to the mean quantity measured in reference blood samples from patients in a state of septic shock when the reference blood samples are taken, or who were in a state of septic shock within 72 h after taking the reference blood samples, and not to have survived.

## Claims

### Claim 1

A kit for the in vitro measurement of a quantity of at least one transcript of the IL7R gene in a biological blood sample, comprising:
specific reagents for measuring the quantity of said at least one transcript of the IL7R gene in said biological blood sample, said at least one transcript comprising the transcript IL7R-001 of SEQ ID NO: 2, or a variant of the transcript IL7R-001 of SEQ ID NO: 2 having at least 99% identity with said sequence, including the following amplification primer pair: forward primer with SEQ ID NO: 12 and reverse primer with SEQ ID NO: 13 and a probe with SEQ ID NO: 14, said probe being either tagged with a marker, or immobilized on a solid support; and
a blood control sample that is a blood sample that is calibrated and contains the quantity of said at least one transcript of the IL7R gene that corresponds to the mean quantity measured in a pool of reference blood samples from human patients who are in a state of septic shock when the reference blood samples are taken, or who were in a state of septic shock within 72 hours after the reference blood samples were taken, and who were known to have survived, and/or a blood control sample that is a blood sample that is calibrated and contains the quantity of said at least one transcript of the IL7R gene that corresponds to the mean quantity measured in a pool of reference blood samples from human patients who are in a state of septic shock when the reference blood samples are taken, or who were in a state of septic shock within 72 hours after the reference blood samples were taken, and who were known not to have survived.

### Claim 2 (depends on 1)

The kit according to claim 1, comprising another blood control sample that is calibrated and contains the quantity of said at least one transcript of the IL7R gene that corresponds to the mean quantity measured in a pool of reference blood samples from human patients who were in a state of septic shock within 24 hours after the reference blood samples were taken, and who were known to have survived, and/or a blood control sample that is a blood sample that is calibrated and contains the quantity of said at least one transcript of the IL7R gene that corresponds to the mean quantity measured in a pool of reference blood samples from human patients who were in a state of septic shock within 24 hours after the reference blood samples were taken, who were known not to have survived.

### Claim 3 (depends on 1)

The kit according to claim 1, further comprising the following amplification primer pair: a forward primer with SEQ ID NO: 15 and a reverse primer with SEQ ID NO: 16.

### Claim 4 (depends on 3)

The kit according to claim 3, further comprising a probe with SEQ ID NO: 17.

### Claim 5 (depends on 1)

The kit according to claim 1, further comprising the following amplification primer pair: a forward primer with SEQ ID NO: 18 and a reverse primer with SEQ ID NO: 19.

### Claim 6 (depends on 5)

The kit according to claim 5, further comprising a probe with SEQ ID NO: 20.