Production of symmetrical bolaform sophorosides
AssigneesUniversiteit Gent, Amphistar
InventorsSophie Roelants, Lisa Van Renterghem, Wim Soetaert, Jelle Remmery
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Abstract
The present invention relates to the field of microbial production of novel biosurfactants. More specifically, the present invention discloses the usage of a fungal strain such as the yeast Starmerella bombicola having a dysfunctional CYP52M1 cytochrome P450 monooxygenase and a dysfunctional FAO1 fatty alcohol oxidase for producing high amounts of so-called “symmetrical bolaform sophorosides” where both sophorose moieties are attached through a terminal glycosidic linkage to the hydrophobic linker. In addition, the present invention further discloses that the latter yeast can also be used to produce alkyl sophorosides and symmetrical bolaform glucosides.
Claims13
A method to produce fully symmetrical bola sophorosides which are free of contaminating fatty acid-containing sophorolipids comprising feeding a mutated fungal strain with a fatty alcohol having an aliphatic tail chain length of at least 6 carbons,
- wherein said fungal strain has a dysfunctional CYP52M1 cytochrome P450 monooxygenase and a dysfunctional FAO1 fatty alcohol oxidase, and
- wherein said fungal strain is Starmerella (Candida) bombicola or a sophorolipid-producing strain of the Starmerella clade.
A mutated fungal strain having a dysfunctional CYP52M1 cytochrome P450 monooxygenase and a dysfunctional FAO1 fatty alcohol oxidase, wherein said fungal strain is a yeast selected from the group consisting of Starmerella (Candida) bombicola, or a sophorolipid-producing strain of the Starmerella clade.
Dependencies are inferred from the claim text.