Engineered methionine gamma lyase variants
AssigneeSyntis Bio, Inc.
InventorsKierra Aleece Franklin, Stephanie Sue Galanie, Gjalt W. Huisman, Nikki D. Kruse, Kerryn McCluskie, Vesna Mitchell, Leann Quertinmont Teadt, Kristen Jean Vallieu
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Abstract
The present invention provides engineered methionine gamma lyase polypeptides and compositions thereof. The engineered methionine gamma lyase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH<7) conditions. The present invention also relates to the use of the compositions comprising the engineered methionine gamma lyase polypeptides for therapeutic purposes.
Claims24
A recombinant methionine gamma lyase comprising a polypeptide sequence comprising at least 85% sequence identity to the reference sequence of SEQ ID NO:
- 1488, wherein the polypeptide sequence comprises at least a substitution 189I/L/M/P/S, 69I/R/W, 237K/A/G/H/L/R/T/Y, 236A/C/R, or 341F, or combinations thereof, wherein the amino acid positions are relative to the reference sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.