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US 12,110,509 B2Granted
Stem cell-derived Sertoli-like cell, preparation method therefor, and use thereof
AssigneeCha University Industry-Academic Cooperation Foundation
InventorsDong Ryul Lee, Dong Won Seol
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Abstract
Provided are stem cells-induced Serotoli-like cells, methods of preparing the same, and uses thereof. Sertoli-like cells according to one embodiment can be differentiated from embryonic stem cells with excellent proliferative capacity, and thus, can be obtained in large quantities. Also, since the Sertoli-like cells secrete immunosuppressive substances and form immune privilege and induce anti-inflammatory functions, they can be used for development of the cell therapeutic agent.
Claims6
A method of preparing Sertoli-like cells (SLC) from stem cells, the method comprising:
- inducing differentiation into intermediate mesoderm (IM) by culturing stem cells in a medium,
- wherein the inducing differentiation into the IM comprises culturing stem cells for one day to three days in the medium comprising a glycogen synthase kinase 3 (GSK-3) inhibitor, and additionally culturing for two days to five days after adding a base fibroblast growth factor (bFGF) and retinoic acid (RA) to the medium;
- and
- inducing differentiation into SLC by culturing the induced IM in a medium comprising bFGF, fibroblast growth factor 9 (FGF9), prostaglandin D2 (PDG2), follicle-stimulating hormone (FSH), and a glial cell-induced neurotrophic factor (GDNF),
- wherein the stem cells are embryonic stem cells (ESC).
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Dependencies are inferred from the claim text.