Pseudotyped insect baculovirus gene transfer system and pseudotyped baculovirus for shrimps, construction method and use thereof

A construction method of a pseudotyped insect baculovirus for shrimps based on a gene transfer system, comprising the following steps:

• 1) constructing, with a Bac-to-Bac insect baculovirus expression system, an insect baculovirus recombinant plasmid comprising a foreign gene;
• and
• 2) co-transfecting the insect baculovirus recombinant plasmid obtained in step 1) and an expression plasmid carrying shrimp virus envelope protein gene into an insect packaging cell to obtain a pseudotyped insect baculovirus for shrimps;
• wherein the gene transfer system for shrimps comprises a Bac-to-Bac insect baculovirus expression system, a shrimp virus envelope protein gene expression plasmid and an insect packaging cell;
• wherein the envelope protein gene in the shrimp virus envelope protein gene expression plasmid has a nucleotide sequence as shown in SEQ ID NO. 1.