# US 12,110,496 B2 · Method of determining the zygosity of event MON 88302

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- Number: US 12,110,496 B2
- Document ID: US12110496B2
- Status: Granted
- Kind: B2
- Application number: 17/517,467
- Filed: 2021-11-02
- Published: 2022-05-19
- Granted: 2024-10-08
- Earliest claimed: 2010-06-04
- Assignees: Monsanto Technology LLC
- Applicants: Monsanto Technology LLC
- Inventors: Andrew J. Brown; James F. Byrne; Robert H. Cole; James H. Crowley; John A. Miklos; Robert C. Ripley; Simone Seifert-Higgins; Jiali Xie
- Web page: https://patentgrep.com/patent/US12110496B2

## CPC

- C12N 15/8275: {Glyphosate}
- A01N 57/20: containing acyclic or cycloaliphatic radicals
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS

## Abstract

The invention provides plants comprising transgenic event MON 88302 that exhibit tolerance to glyphosate herbicide. The invention also provides seeds, plant parts, cells, commodity products, and methods related to the event. The invention also provides DNA molecules that are unique to the event and were created by the insertion of transgenic DNA into the genome of a Brassica napus plant.

## Claims

### Claim 1

A method of determining the zygosity of a Brassica napus plant or seed comprising event MON 88302, wherein a representative sample of seed comprising said event has been deposited under ATCC Accession Number PTA-10955, said method comprising:
a) contacting a sample comprising DNA with a primer set comprising SEQ ID NO: 12, SEQ ID NO: 13, and SEQ ID NO: 14, that when used in a nucleic acid amplification reaction with genomic DNA from event MON 88302 produces a first amplicon that is diagnostic for event MON 88302, and that when used in a nucleic acid amplification reaction with genomic DNA from a plant that does not comprise event MON 88302 produces a second amplicon comprising the native genomic DNA region spanning the insertion location of event MON 88302;
b) performing a nucleic acid amplification reaction, thereby producing said first amplicon, said second amplicon, or both said first amplicon and said second amplicon; and
c) detecting said first amplicon, said second amplicon, or both said first amplicon and said second amplicon, wherein the presence of only said first amplicon indicates that said sample is homozygous for event MON 88302, and wherein the presence of both said first amplicon and said second amplicon indicates that the sample is heterozygous for event MON 88302.

### Claim 2 (depends on 1)

The method of claim 1, comprising determining the zygosity of a Brassica napus plant comprising event MON 88302.

### Claim 3 (depends on 1)

The method of claim 1, comprising determining the zygosity of a Brassica napus seed comprising event MON 88302.

### Claim 4 (depends on 1)

The method of claim 1, wherein detecting said first amplicon, said second amplicon, or both said first amplicon and said second amplicon is by agarose gel electrophoresis.