# US 12,110,485 B2 · Leuconostoc citreum and use thereof in precipitating starch emulsion

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 12,110,485 B2
- Document ID: US12110485B2
- Status: Granted
- Kind: B2
- Application number: 17/312,013
- Filed: 2020-11-24
- Published: 2023-08-10
- Granted: 2024-10-08
- Earliest claimed: 2020-04-29
- Assignees: China Agricultural University; Sishui Lifeng Food Co., Ltd.
- Applicants: China Agricultural University; Sishui Lifeng Food Co., Ltd.
- Inventors: Xiaojun Liao; Xuan Wang; Liang Zhao; Yongtao Wang; Xiankui Kong; Hongyan Chen; Xueqiang Wang; Guanghua Li
- Web page: https://patentgrep.com/patent/US12110485B2

## CPC

- C12N 1/20: Bacteria; Culture media therefor
- C08B 30/04: Extraction or purification
- C12N 1/205: Bacterial isolates
- C12P 19/04: Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C08B: POLYSACCHARIDES; DERIVATIVES THEREOF (polysaccharides containing less than six saccharide radicals attached to each other by glycosidic linkages C07H; fermentation or enzyme-using processes C12P19/00; sugar industry C13; production of cellulose D21)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

Provided is a method for precipitating a starch emulsion, the method including: mixing a fermentation broth with the starch emulsion, the fermentation broth including an isolated microorganism, wherein the isolated microorganism is Leuconostoc citreum WSJ-57 deposited under an accession number of CGMCC No. 19201 in the China General Microbiological Culture Collection Center on Dec. 20, 2019.

## Claims

### Claim 1

A method for precipitating a starch emulsion, the method comprising:
mixing (i) a fermentation broth containing a culture of Leuconostoc citreum WSJ-57, deposited under accession number CGMCC No. 19201, with (ii) a starch emulsion, to produce a mixture; and
performing stand treatment of the mixture sufficient to precipitate the starch emulsion.

### Claim 2 (depends on 1)

The method according to claim 1, wherein a concentration of the Leuconostoc citreum WSJ-57 in the fermentation broth ranges from 1×109 CFU/mL to 5×109 CFU/mL, a starch concentration of the starch emulsion ranges from 10 g/L to 40 g/L, and a volume ratio of the fermentation broth to the starch emulsion ranges from 1:10 to 1:15.