Enzymatic synthesis of soluble glucan fiber
AssigneeNutrition & Biosciences USA 4, Inc.
InventorsQiong Cheng, Robert DiCosimo, Arthur Ouwehand, Zheng You, Mark S. Payne, Jian Ping Lai, Kristin Ruebling-Jass, Steven Cary Rothman
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Abstract
An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.
Claims20
A method of producing alpha-glucan, said method comprising:
- combining a food that has at least water and sucrose with a glucosyltransferase enzyme comprising an amino acid sequence that is at least 91% identical to SEQ ID NO:
- 10,
- wherein alpha-glucan comprising 100% alpha-1,6 glycosidic linkages is produced by the glucosyltransferase enzyme in the food and the method increases the amounts of soluble alpha-glucan and fructose in the food as measured.
A method of producing alpha-glucan, said method comprising:
- combining a food that has at least water and sucrose with a glucosyltransferase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:43,
- wherein the food is a dairy product, carbonated beverage, fruit juice, bakery product, or is selected from the group consisting of yogurt, yogurt drinks, milk drinks, flavored milks, smoothies, ice cream, shakes, quarg, whipped mousse, fondants, nougats, soups, sauces, dressings, and coffee creamers,
- wherein alpha-glucan is produced by the glucosyltransferase enzyme in the food and the method increases the amounts of soluble alpha-glucan and fructose in the food as measured.
Dependencies are inferred from the claim text.