Binding proteins and chimeric antigen receptor T cells targeting GASP-1 granules and uses thereof

A method for detecting granules expressing G protein coupled receptor-associated sorting protein 1 (GASP-1) in cells, comprising:

• (a) contacting the cells with an effective amount of a binding protein, wherein the binding protein comprises an antigen binding fragment that specifically binds the GASP-1, wherein the binding protein comprises an anti-GASP-1 single-chain variable fragment (anti-GASP-1 scFv), whereby the binding protein is bound to the GASP-1, wherein the anti-GASP-1 scFv comprises a variable heavy (VH) chain and a variable light (VL) chain, and wherein the VH chain comprises an amino acid sequence of SEQ ID NO:
• 1 and the VL chain comprises SEQ ID NO:
• 9 or 17, or wherein the VH chain comprises the amino acid sequence of SEQ ID NO:
• 2, the amino acid sequence of SEQ ID NO:
• 3 and the amino acid sequence of SEQ ID NO:
• 4, and the VL chain comprises the amino acid sequence of SEQ ID NO:
• 10 or 18, the amino acid sequence of SEQ ID NO:
• 11, and the amino acid sequence of SEQ ID NO:
• 12;
• and
• (b) identifying the binding protein of step (a) in granules bound to a membrane of the cells, wherein the granules express the GASP-1 in the cells.