# US 12,110,315 B1 · Fish-derived peptidoglycan recognition protein mutant and application thereof

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- Number: US 12,110,315 B1
- Document ID: US12110315B1
- Status: Granted
- Kind: B1
- Application number: 18/607,763
- Filed: 2024-03-18
- Published: 2024-09-26
- Granted: 2024-10-08
- Earliest claimed: 2023-03-21
- Assignees: Ocean University of China
- Applicants: Ocean University of China
- Inventors: Qingfei Li; Qinghui Ai
- Web page: https://patentgrep.com/patent/US12110315B1

## CPC

- C07K 14/461: {from fish}
- A61P 31/04: Antibacterial agents
- A61P 37/04: Immunostimulants
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

A fish-derived peptidoglycan recognition protein mutant and an application thereof are provided. An amino acid sequence of the mutant is shown in SEQ ID NO. 1, a nucleotide sequence of the mutant is shown in SEQ ID NO. 2. The present disclosure provides the preparation method for the mutant with a broad-spectrum antibacterial activity and robust inhibitory effects on both Gram-positive and Gram-negative bacteria. Compared with the wild-type LcPGRP5 protein, the mutant protein demonstrates a stronger ability to inhibit the activity against Staphylococcus aureus and enhance the phagocytic ability of fish macrophages.

## Claims

### Claim 1

A fish-derived peptidoglycan recognition protein mutant, wherein the amino acid sequence of the mutant is shown in SEQ ID NO. 1, the nucleotide sequence of coding gene of the mutant is shown in SEQ ID NO. 2.