Engineered bacterial ribosome compositions and methods
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsSidney Hecht, Larisa Dedkova, Shengxi Chen, Xiaoguang Bai
Life of this patent
Estimating the term…
Abstract
Compositions and methods relating to bacterial ribosomes selected to increase the incorporation of at least one glycosylated amino acid into a protein versus a wild-type bacterial ribosome. Selection embodiments include growing bacteria in the presence of a puromycin derivative, wherein a surviving clone has a ribosome that incorporates at least one glycosylated amino acid into a protein.
Claims11
An Escherichia coli (E. coli) bacterial ribosome comprising a modified 23S ribosomal RNA sequence comprising modifications as compared to a wild-type counterpart, wherein the modifications consist of a first modified region and a second modified region;
- wherein the 23S ribosomal RNA is expressed from an engineered rrnB operon;
- wherein the first modified region consists of the sequence UGCGUGG from position 2057 to 2063;
- wherein the second modified region consists of a sequence selected from GGGAAG and UCGAGA from position 2496 to 2501;
- wherein a DNA sequence encoding a first unmodified region of the wild-type counterpart consists of the sequence GAAAGAC from position 2057 to 2063;
- and
- wherein a second unmodified region of the wild-type counterpart consists of the sequence CACCUC from position 2496 to 2501.
Dependencies are inferred from the claim text.