# US 12,109,563 B2 · Test cartridge for analysis instruments > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,109,563 B2 - Document ID: US12109563B2 - Status: Granted - Kind: B2 - Application number: 17/309,918 - Filed: 2020-09-29 - Published: 2022-03-10 - Granted: 2024-10-08 - Earliest claimed: 2019-09-30 - Assignees: Illumina Singapore Pte. Ltd. - Applicants: Illumina Singapore Pte. Ltd. - Inventors: Beng Keong Ang; Shyun Long Wang; Heng Kuang Cheng; Chow Jin Chng; Ai Wee Lee; Yashwanth Panduga - Web page: https://patentgrep.com/patent/US12109563B2 ## CPC - B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces} - B01L 3/502738: {characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746)} - B01L 7/00: Heating or cooling apparatus (autoclaves B01J3/04); Heat insulating devices - G01N 35/1097: {characterised by the valves (valves in general F16K)} - B01L 2200/146: Employing pressure sensors - B01L 2200/147: Employing temperature sensors - B01L 2300/0627: Sensor or part of a sensor is integrated - B01L 2300/0663: Whole sensors - B01L 2300/18: Means for temperature control - B01L 2400/0478: pistons - B01L 2400/0487: fluid pressure, pneumatics - B01L 2400/0644: rotary valves - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract A test cartridge for insertion into an analysis instrument having an actuation mechanism, the test cartridge having one or more sensors for measuring an aspect of the actuation mechanism. ## Claims ### Claim 1 A test cartridge for insertion into an analysis instrument having a linear actuation mechanism, a rotational actuation mechanism, a heater, a pump actuation mechanism, a second linear actuation mechanism, and a cartridge receiver, the test cartridge comprising: a test cartridge housing having a form factor configured to be received by the cartridge receiver of the analysis instrument; a plurality of first linear translation sensors, each first linear translation sensor having a corresponding first detector and a corresponding first movable element linearly translatable along a corresponding first axis, wherein at least a first portion of each of the first linear translation sensors is positioned within the test cartridge housing such that the corresponding first movable element thereof is configured to engage with the linear actuation mechanism of the analysis instrument when the test cartridge is inserted into the analysis instrument and the linear actuation mechanism of the analysis instrument is actuated, and wherein the first detector of each of the first linear translation sensors is configured to detect a first distance moved by the corresponding first movable element along the corresponding first axis relative to the test cartridge housing and responsive to a linear input provided by the linear actuation mechanism of the analysis instrument when the test cartridge is inserted into the analysis instrument and the linear actuation mechanism of the analysis instrument is actuated; a second linear translation sensor having a second detector and a second movable element linearly translatable along a second axis, wherein at least a second portion of the second linear translation sensor is positioned within the test cartridge housing such that the second movable element is configured to engage with the pump actuation mechanism of the analysis instrument when the test cartridge is inserted into the analysis instrument and the pump actuation mechanism of the analysis instrument is actuated, and wherein the second detector is configured to detect a second distance moved by the second movable element along the second axis relative to the test cartridge housing when the test cartridge is inserted into the analysis instrument and the pump actuation mechanism of the analysis instrument is actuated; an angular rotation sensor having a third detector and a rotatable element rotatable about a third axis, wherein the angular rotation sensor is positioned within the test cartridge housing such that the rotatable element is configured to engage with the rotational actuation mechanism of the analysis instrument when the test cartridge is inserted into the analysis instrument and the rotational actuation mechanism of the analysis instrument is actuated, and wherein the third detector is configured to detect an angle moved by the rotatable element about the third axis relative to the test cartridge housing when the test cartridge is inserted into the analysis instrument and the rotational actuation mechanism of the analysis instrument is actuated; a temperature sensor having a receptacle in the test cartridge housing and a temperature detector positioned within the receptacle, wherein the temperature sensor is positioned within the test cartridge housing such that the receptacle is configured to engage with the heater of the analysis instrument when the test cartridge is inserted into the analysis instrument, and wherein the temperature detector is configured to detect a temperature inside the receptacle when the test cartridge is inserted into the analysis instrument; and a plurality of load cells, wherein each of the load cells is positioned within the test cartridge housing such that each load cell is configured to engage with the second linear actuation mechanism of the analysis instrument when the test cartridge is inserted into the analysis instrument and the second linear actuation mechanism of the analysis instrument is actuated, and wherein each of the load cells is configured to detect a force applied to the respective load cell when the test cartridge is inserted into the analysis instrument and the second linear actuation mechanism of the analysis instrument is actuated. ### Claim 2 (depends on 1) The test cartridge of claim 1, further comprising a controller having one or more processors and one or more memory devices, wherein: the controller is communicatively connected with the first detector of each first linear translation sensor, the second detector of the second linear translation sensor, the third detector of the angular rotation sensor, the temperature detector of the temperature sensor, and each of the load cell, and the controller is configured to receive and store data generated by each first detector, the second detector, the third detector, the temperature sensor, and each of the load cells. ### Claim 3 (depends on 2) The test cartridge of claim 2, wherein: the controller is configured to cause the transmission of updated movement data to the analysis instrument, and the updated movement data is based, at least in part, on one or more of data generated by one or more of the first detectors, the second detector, the third detector, the temperature sensor, and each of the load cells. ### Claim 4 (depends on 2) The test cartridge of claim 2, wherein: the controller is configured to cause the transmission of the data generated by the first detectors, the second detector, the third detector, the temperature sensor, and each of the load cells to the analysis instrument, and the analysis instrument is configured to issue a notification based, at least in part, on the data generated by the first detectors, the second detector, the third detector, the temperature sensor, and each of the load cells. ### Claim 5 (depends on 1) The test cartridge of claim 1, further comprising a communications interface configured to transmit data generated by each of the first linear translation sensors, the second linear translation sensor, the angular rotation sensor, the temperature sensor, and the load cells to an external device. ### Claim 6 (depends on 1) The test cartridge of claim 1 wherein: each of the first linear translation sensors further includes a corresponding first resistive element configured to apply a first resistive force against the corresponding first movable element of that first linear translation sensor along the corresponding first axis of that first linear translation sensor, and the angular rotation sensor further includes a second resistive element configured to apply a second resistive force against the rotatable element. ### Claim 7 (depends on 1) The test cartridge of claim 1, wherein: the angular rotation sensor further includes a friction element configured to contact and apply a frictional resistance force against the rotatable element, the angular rotation sensor further includes a resistive element positioned such that the friction element is interposed between the resistive element and the rotatable element, and the resistive element is configured to apply a force to the friction element along the third axis and in a direction towards the rotatable element. ### Claim 8 (depends on 1) The test cartridge of claim 1, further comprising a second angular rotation sensor having a second angular detector and a second rotatable element rotatable about a second rotation axis, wherein the second angular rotation sensor is positioned such that the second rotatable element is configured to engage with a second rotational actuation mechanism of the analysis instrument when the test cartridge is inserted into the analysis instrument and the second rotational actuation mechanism of the analysis instrument is actuated, and wherein the second angular detector is configured to detect a second angle moved by the second rotatable element about the second rotation axis relative to the test cartridge housing when the test cartridge is inserted into the analysis instrument and the second rotational actuation mechanism of the analysis instrument is actuated. ### Claim 9 A test cartridge for insertion into an analysis instrument having a linear actuation mechanism and a cartridge receiver, the test cartridge comprising: a test cartridge housing having a form factor configured to be received by the cartridge receiver of the analysis instrument; and a plurality of linear translation sensors, each of the linear translation sensors having a corresponding first detector and a corresponding first movable element linearly translatable along a corresponding first axis, wherein at least a portion of each of the linear translation sensors is positioned within the test cartridge housing of the test cartridge such that the corresponding first movable element thereof is configured to engage with the linear actuation mechanism of the analysis instrument when the test cartridge is inserted into the analysis instrument and the linear actuation mechanism of the analysis instrument is actuated, and each first detector is configured to detect a distance moved by the corresponding first movable element along the corresponding first axis relative to the test cartridge housing when the test cartridge is inserted into the analysis instrument and the linear actuation mechanism of the analysis instrument is actuated. ### Claim 10 (depends on 9) The test cartridge of claim 9, wherein each linear translation sensor further includes a corresponding contact portion configured to be contacted by an engagement pin of the linear actuation mechanism. ### Claim 11 (depends on 9) The test cartridge of claim 9, wherein: each first movable element includes an encoder strip, and each first detector is an optical sensor configured to detect a movement of the encoder strip included in the corresponding first movable element. ### Claim 12 (depends on 9) The test cartridge of claim 9, further comprising a controller having one or more processors and one or more memory devices, wherein: the controller is communicatively connected with each of the first detectors, and the controller is configured to receive and store data generated by the first detectors. ### Claim 13 (depends on 12) The test cartridge of claim 12, further comprising a communications interface to transmit data generated by each of the linear translation sensors, wherein: the communications interface is configured to transmit data generated by each of the linear translation sensors to the analysis instrument, the controller is configured to cause the transmission of calibration data to the analysis instrument, and the calibration data is based, at least in part, data generated by one or more linear translation sensors. ### Claim 14 A test cartridge for insertion into an analysis instrument having a rotational actuation mechanism, a heater, and a cartridge receiver, the test cartridge comprising: a test cartridge housing having a form factor configured to be received by the cartridge receiver of the analysis instrument and a port configured to receive the heater of the analysis instrument; a first angular rotation sensor having a first detector and a first rotatable element rotatable about a first rotation axis, wherein the first angular rotation sensor is positioned within the test cartridge housing such that the first rotatable element is configured to engage with the rotational actuation mechanism of the analysis instrument when the test cartridge is inserted into the analysis instrument and the rotational actuation mechanism of the analysis instrument is actuated, and wherein the first detector is configured to detect a first angle moved by the first rotatable element about the first rotation axis relative to the test cartridge housing when the test cartridge is inserted into the analysis instrument and the rotational actuation mechanism of the analysis instrument is actuated; and a temperature sensor having a receptacle in the test cartridge housing and a temperature detector positioned within the receptacle, wherein the temperature sensor is positioned within the test cartridge housing such that the receptacle is configured to engage with the heater of the analysis instrument when the test cartridge is inserted into the analysis instrument, wherein the receptacle is configured to be heated by the heater of the analysis instrument, and wherein the temperature detector is configured to detect a temperature inside the receptacle. ### Claim 15 (depends on 14) The test cartridge of claim 14, wherein the first rotatable element includes a shaft and features configured to engage with the rotational actuation mechanism of the analysis instrument. ### Claim 16 (depends on 14) The test cartridge of claim 14, wherein: the first angular rotation sensor further includes an encoder disk having a disk center axis that is collinear with a center axis of the first rotatable element and the first axis, and the first detector is an optical sensor configured to detect a movement of encoder disk. ### Claim 17 (depends on 14) The test cartridge of claim 14, wherein the receptacle is configured to be contacted by the heater of the analysis instrument when the test cartridge is inserted into the analysis instrument and the heater is inserted through the port. ### Claim 18 (depends on 14) The test cartridge of claim 14, further comprising a torque sensor configured to measure a torque applied to the rotatable element. ### Claim 19 (depends on 14) The test cartridge of claim 14, further comprising a controller having one or more processors and one or more memory devices, wherein: the controller is communicatively connected with the first detector and the temperature detector, and the controller is configured to receive and store data generated by the first detector and the temperature detector. ### Claim 20 (depends on 19) The test cartridge of claim 19, further comprising a communications interface, wherein: the communications interface is configured to transmit data generated by the first angular rotation sensor and the temperature sensor, the controller is configured to cause the transmission of data generated by the first angular rotation sensor and the temperature sensor to the analysis instrument, and the analysis instrument is configured to issue a notification based, at least in part, on data generated by one or more of the first angular rotation sensor and the temperature sensor. ### Claim 21 (depends on 5) The test cartridge of claim 5, wherein the external device is the analysis instrument. ### Claim 22 (depends on 9) The test cartridge of claim 9, wherein each linear translation sensor further includes a resistive element configured to apply a resistive force against the corresponding first movable element along the corresponding first axis and in a corresponding first direction. ### Claim 23 (depends on 22) The test cartridge of claim 22, wherein the resistive element is a compression spring. ### Claim 24 A test cartridge for insertion into an analysis instrument having a pump actuation mechanism and a cartridge receiver, the test cartridge comprising: a test cartridge housing having a form factor configured to be received by the cartridge receiver of the analysis instrument, wherein the test cartridge includes a first hole and a second hole opposite the first hole, and the second hole is configured to receive a portion of the pump actuation mechanism of the analysis instrument; and a linear translation sensor having a detector and a movable element linearly translatable along a first axis, wherein: at least a portion of the movable element is positioned within the test cartridge housing of the test cartridge, the movable element is positioned in between the first hole and the second hole, the movable element is configured to engage with and is movable by the portion of the pump actuation mechanism received by the second hole when the test cartridge is inserted into the analysis instrument and the pump actuation mechanism of the analysis instrument is actuated, the movable element is configured to be movable through the first hole when the test cartridge is inserted into the analysis instrument and the pump actuation mechanism of the analysis instrument is actuated to move the movable element, and the detector is configured to detect a distance moved by the movable element along the first axis relative to the test cartridge housing. ### Claim 25 (depends on 24) The test cartridge of claim 24, further comprising a controller having one or more processors and one or more memory devices, wherein: the controller is communicatively connected with the detector of the linear translation sensor, and the controller is configured to receive and store data generated by the detector of the linear translation sensor. ### Claim 26 (depends on 24) The test cartridge of claim 24, further comprising a communications interface configured to transmit data generated by the linear translation sensor. ### Claim 27 (depends on 14) The test cartridge of claim 14, further comprising a second angular rotation sensor having a second detector and a second rotatable element rotatable about a second rotation axis, wherein the second angular rotation sensor is positioned within the test cartridge housing such that the second rotatable element is configured to engage with a second rotational actuation mechanism of the analysis instrument when the test cartridge is inserted into the analysis instrument and the second rotational actuation mechanism of the analysis instrument is actuated, and wherein the second detector is configured to detect a second angle moved by the second rotatable element about the second rotation axis relative to the test cartridge housing when the test cartridge is inserted into the analysis instrument and the second rotational actuation mechanism of the analysis instrument is actuated. ### Claim 28 (depends on 27) The test cartridge of claim 27, wherein the first rotation axis and second rotation axis are parallel to each other. ### Claim 29 (depends on 14) The test cartridge of claim 14, wherein the first angular rotation sensor further includes a resistive element configured to apply a resistive force against the first rotatable element. ### Claim 30 (depends on 14) The test cartridge of claim 14, wherein the first angular rotation sensor further includes a friction element configured to contact and apply a frictional resistance force against the first rotatable element. ### Claim 31 (depends on 30) The test cartridge of claim 30, wherein: the first angular rotation sensor further includes a resistive element positioned such that the friction element is interposed between the resistive element and the first rotatable element, and the resistive element is configured to apply a force to the friction element along the rotation axis in a direction towards the first rotatable element. ### Claim 32 (depends on 14) The test cartridge of claim 14, wherein the receptacle is comprised of a metal. ### Claim 33 (depends on 17) The test cartridge of claim 17, wherein: the receptacle further includes a heating collar around the receptacle, and the heating collar is configured to be contacted by the heater of the analysis instrument when the test cartridge is inserted into the analysis instrument and the heater is inserted through the port.