Preparation method of AM-type polystyrene microsphere ofloxacin imprinted polymer and application thereof

A preparation method of AM-type polystyrene microsphere ofloxacin imprinted polymer, comprising:

• (1) activating polystyrene primary amine resin microspheres by:
• immersing polystyrene primary amine resin microspheres in a N,N-dimethyl formamide solution for 6-8 h, washing the polystyrene primary amine resin microspheres with distilled water sufficiently, and drying in vacuum for 20-28 hours until constant weight, to thereby obtain activated polystyrene primary amine resin microspheres;
• (2) grafting of AM on polystyrene primary amine resin microspheres by:
• adding 0.3-0.5 g the activated polystyrene primary amine resin microspheres into a four-neck flask equipped with an electric stirrer and a reflux condenser, subsequently adding 8-12 mL acrylamide monomer and a solvent ethyl alcohol, then removing nitrogen, and heating;
• after reaching 25-35° C., adding an initiator ammonium persulfate, reacting at a temperature condition of 25-35° C. for 6-10 h until termination;
• washing the microspheres with distilled water repeatedly and drying in a vacuum until constant weight, to thereby obtain grafted particles PAM/PSA;
• and
• (3) preparing surface molecularly imprinted materials:
• the grafted particles PAM/PSA generate strong adsorption to levofloxacin to reach adsorption equilibrium, then with use of a surface imprinting technology, employing saturated adsorption AM grafted polystyrene primary amine resin microspheres, adding an ethyl alcohol solution and 0.005 g/L of levofloxacin solution as a solvent, and adding ethylene glycol diglycidyl ether as the crosslinking agent to thereby prepare a levofloxacin surface molecularly imprinted material MIP-PAM/PSA.