Exosome extraction device and exosome extraction method

An exosome extraction device comprising:

• a stock solution supply unit supplying an exosome-containing liquid comprising a cell culture supernatant containing exosomes;
• a first storage unit fluidly connected to the stock solution supply unit, the first storage unit storing, in an inside thereof, the exosome-containing liquid supplied from the stock solution supply unit;
• a positive pressure load unit fluidly connected to the first storage unit and configured for applying a positive pressure to the inside of the first storage unit;
• a first filter provided between the stock solution supply unit and the first storage unit and having a hole diameter that passes exosomes and blocks cells, the exosome extraction device being configured to supply the exosome-containing liquid from the stock solution supply unit to the first storage unit through the first filter;
• a second filter having a hole diameter that blocks exosomes, the second filter having a pre-filtration chamber and a post-filtration chamber;
• a circulation path comprising a supply path and a return path fluidly interconnecting the first storage unit and the pre-filtration chamber of the second filter, the exosome extraction device being configured to circulate the exosome-containing liquid stored in the first storage unit between the first storage unit and the second filter, so that the exosome-containing liquid flows from the first storage unit through the supply path to the pre-filtration chamber, and then from the pre-filtration chamber through the return path and back to the first storage unit to concentrate the exosome-containing liquid, the post-filtration chamber collecting and storing filtered liquid from the exosome-containing liquid;
• a discharge path fluidly connected to the post-filtration chamber of the second filter for discharging the filtered liquid from the post-filtration chamber;
• a second storage unit fluidly connected to the post-filtration chamber of the second filter through the discharge path;
• a recovery unit fluidly connected to the first storage unit for recovering the concentrated exosome-containing liquid stored in the first storage unit;
• a third filter provided between the first storage unit and the recovery unit and having a hole diameter that passes exosomes and blocks bacteria, the exosome extraction device being configured to supply the concentrated exosome-containing liquid, returned to the first storage unit from the pre-filtration chamber via the return path and stored in the first storage unit, to the recovery unit through the third filter;
• a steam supply unit fluidly connect to the first storage unit, the exosome extraction device being configured to supply steam from the steam supply unit to the first storage unit and from the first storage unit to the first filter and the third filter to sterilize the first filter and the third filter;
• and
• a chemical supply unit fluidly connected to the second storage unit, the exosome extraction device being configured to supply a cleaning chemical agent from the chemical supply unit to the second storage unit and from the second storage unit to the second filter to clean the second filter.