# US 12,109,316 B2 · Deoxyribonucleic acid nanoshells for co-delivering proteins and protein-containing compounds and methods of manufacturing deoxyribonucleic acid nanoshells containing proteins and protein-containing compounds

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- Number: US 12,109,316 B2
- Document ID: US12109316B2
- Status: Granted
- Kind: B2
- Application number: 17/076,435
- Filed: 2020-10-21
- Published: 2021-02-04
- Granted: 2024-10-08
- Earliest claimed: 2016-12-02
- Assignees: Howard University
- Applicants: Howard University
- Inventors: Preethi Chandran
- Web page: https://patentgrep.com/patent/US12109316B2

## CPC

- A61K 9/5146: {obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, polyamines, polyanhydrides}
- A61K 9/0019: {Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner (non-active ingredients are additionally classified in A61K47/00)}
- A61K 9/513: {Organic macromolecular compounds; Dendrimers}
- A61K 9/5161: {Polysaccharides, e.g. alginate, chitosan, cellulose derivatives; Cyclodextrin}
- A61K 9/5192: {Processes}
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- A61K 47/549: {Sugars, nucleosides, nucleotides or nucleic acids}
- A61K 47/59: obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
- A61K 47/6925: {the form being a microcapsule, nanocapsule, microbubble or nanobubble}
- C08G 73/02: Polyamines
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C08G: MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS

## Abstract

Non-viral delivery platforms are provided for facilitating transport of molecules across cell membranes. In some forms, DNA nanoshells capable of transporting cargo molecules are formed, and may be formed in order to surround a variety of materials for a variety of purposes.

## Claims

### Claim 1

A method for protecting toxic or enzymatic proteins from an environment, the method comprising combining a deoxyribonucleic acid (DNA) and a glycosylated polymer in solution separated from the environment to form a nanoshell precursor, adding the toxic or enzymatic protein to the solution before the nanoshell precursor has fully formed into a nanoshell, and thereafter introducing the fully formed nanoshell into the environment.

### Claim 2 (depends on 1)

The method of claim 1, wherein the nanoshell forms an interior space and the protein is disposed within the interior space.

### Claim 3 (depends on 1)

The method of claim 1, wherein the nanoshell has a diameter of from about 50 nm to about 250 nm.

### Claim 4 (depends on 1)

The method of claim 1, wherein the glycosylated polymer comprises polyethylenimine.

### Claim 5 (depends on 1)

The method of claim 1, wherein the glycosylated polymer comprises mannobiose or mannose.

### Claim 6 (depends on 1)

The method of claim 1, wherein the nanoshell comprises a glycan shell on at least an exterior surface thereof.

### Claim 7 (depends on 6)

The method of claim 6, wherein the nanoshell further comprises a glycan shell on an interior surface of the nanoshell.