# US 12,109,300 B2 · Method for preparing enzyme-treated Zizania latifolia Turcz. extract having increased tricin content, and composition for whitening, wrinkle reduction, anti-inflammation, anti-allergy and moisturization, prepared thereby

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- Number: US 12,109,300 B2
- Document ID: US12109300B2
- Status: Granted
- Kind: B2
- Application number: 17/049,025
- Filed: 2019-09-03
- Published: 2021-08-19
- Granted: 2024-10-08
- Earliest claimed: 2018-12-12
- Assignees: Btc Corporation
- Applicants: Btc Corporation
- Inventors: Tae Young Kim; Joo Myung Moon; Sung Kwan Jo
- Web page: https://patentgrep.com/patent/US12109300B2

## CPC

- A61K 8/9794: Liliopsida [monocotyledons]
- A23L 29/06: {Enzymes}
- A23L 33/105: Plant extracts, their artificial duplicates or their derivatives
- A23L 33/40: {Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula}
- A61K 8/498: {having 6-membered rings or their condensed derivatives, e.g. coumarin}
- A61K 31/353: 3,4-Dihydrobenzopyrans, e.g. chroman, catechin
- A61K 36/899: Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61P 29/00: Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
- A61P 37/08: Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14)
- A61Q 19/02: for chemically bleaching or whitening the skin
- A61Q 19/08: Anti-ageing preparations
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A61K 2236/19: involving fermentation using yeast, bacteria or both; enzymatic treatment
- A61K 2236/333: using mixed solvents, e.g. 70% EtOH
- A61K 2800/805: Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

The present invention relates to a method of preparing an enzyme-treated Zizania latifolia Turcz. extract and a composition prepared therefrom, and the enzyme-treated Zizania latifolia Turcz. extract prepared by the above method contains a high content of tricin, and thus it can be usefully used as a cosmetic composition or a health functional food composition for whitening, wrinkle improvement, anti-inflammatory, anti-allergy and moisturizing.

## Claims

### Claim 1

A method of enhancing skin activities, comprising administering an effective amount of a cosmetic composition comprising an enzyme-treated Zizania latifolia Turcz. extract prepared by the method comprising the steps of:
(1) adding water to Zizania latifolia Turcz. to produce a solution and heating for a sufficient duration of time;
(2) cooling heated solution and treating with one or more enzyme(s) selected from the group consisting of: α-amylase, β-galactosidase, xylanase, lactase, lipase, tannase, cellulase, hemicellulase, pectinase and β-glucosidase, to produce an enzyme-treated solution;
(3) filtering the enzyme-treated solution to obtain a primary extract and a remaining residue;
(4) extracting the remaining residue after filtering in step (3) with any solvent selected from the group consisting of: water, lower alcohols of C1 to C4 and mixtures thereof to obtain a secondary extract; and
(5) mixing the primary and secondary extracts and concentrating or drying to produce the enzyme-treated Zizania latifolia Turcz. extract;
wherein the enzyme-treated Zizania latifolia Turcz. extract contains 0.01% to 90% by weight of tricin.

### Claim 2 (depends on 1)

The method of claim 1, wherein the skin activities are selected from the group consisting of skin whitening, moisturizing, anti-inflammatory, anti-allergy and anti-wrinkle.

### Claim 3

A method of enhancing skin activities, comprising administering an effective amount of a health functional food comprising an enzyme-treated Zizania latifolia Turcz. extract prepared by the method comprising the steps of:
(1) adding water to Zizania latifolia Turcz. to produce a solution and heating for a sufficient duration of time;
(2) cooling heated solution and treating with one or more enzyme(s) selected from the group consisting of: α-amylase, β-galactosidase, xylanase, lactase, lipase, tannase, cellulase, hemicellulase, pectinase and β-glucosidase, to produce an enzyme-treated solution;
(3) filtering the enzyme-treated solution to obtain a primary extract and a remaining residue;
(4) extracting the remaining residue after filtering in step (3) with any solvent selected from the group consisting of: water, lower alcohols of C1 to C4 and mixtures thereof to obtain a secondary extract; and
(5) mixing the primary and secondary extracts and concentrating or drying to produce the enzyme-treated Zizania latifolia Turcz. extract;
wherein the enzyme-treated Zizania latifolia Turcz. extract contains 0.01% to 90% by weight of tricin.

### Claim 4 (depends on 3)

The method of claim 3, wherein the skin activities are selected from the group consisting of skin whitening, moisturizing, anti-inflammatory, anti-allergy and anti-wrinkle.