Method for producing artificial recombinant rotavirus
AssigneeOsaka University
InventorsTakeshi Kobayashi, Yuta Kanai
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Abstract
Provided is a method for producing an artificial recombinant virus of the family Reoviridae, the method comprising the steps of: (1) introducing a FAST protein expression vector and/or a capping enzyme expression vector into host cells; (2) introducing a vector containing expression cassettes for individual RNA genome segments of a virus or introducing a set of single-stranded RNA transcripts from the expression cassettes into host cells; and (3) culturing the host cells. The method of the present invention allows more efficient production of an artificial recombinant virus of the family Reoviridae as compared with conventional methods and allows artificial recombinant rotavirus production without using a helper virus.
Claims15
A method for producing an artificial recombinant virus of the family Reoviridae, the method comprising:
- (1) introducing a FAST protein expression vector into host cells;
- (2) introducing a vector containing a set of expression cassettes for individual RNA genome segments of a virus or introducing a set of single-stranded RNA transcripts of the RNA genome segments from the expression cassettes into host cells;
- and
- (3) culturing the host cells.
A method for promoting viral replication, comprising infecting host cells expressing a FAST protein with a virus of the family Reoviridae and culturing the host cells.
Dependencies are inferred from the claim text.