# US 12,109,247 B2 · Antimicrobial peptides

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 12,109,247 B2
- Document ID: US12109247B2
- Status: Granted
- Kind: B2
- Application number: 17/932,316
- Filed: 2022-09-15
- Published: 2023-02-23
- Granted: 2024-10-08
- Earliest claimed: 2017-07-14
- Assignees: MATERIAS S.r.l.
- Applicants: MATERIAS S.r.l.
- Inventors: Marco Balestrieri; Gianna Palmieri; Gianluca Neglia; Aniello Anastasio; Federico Capuano; Luca De Stefano; Luigi Nicolais
- Web page: https://patentgrep.com/patent/US12109247B2

## CPC

- A61K 38/10: Peptides having 12 to 20 amino acids {(A61K38/043 - A61K38/046 take precedence)}
- A01N 37/46: N-acyl derivatives
- A01N 63/50: Isolated enzymes; Isolated proteins (peptides A01N37/46)
- A61K 47/6929: {the form being a nanoparticle, e.g. an immuno-nanoparticle}
- A61P 31/04: Antibacterial agents
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention relates to the use of at least one peptide having sequence VRLIVAVRIWRR, VRLIVAVRIKRR, VRLIVKVRIWRR, VRLIVKVRIKRR (SEQ ID NO: 1, SEQ ID NO 2, SEQ ID NO 3, SEQ ID NO 4), as a bactericidal antimicrobial agent against Listeria monocytogenes.

## Claims

### Claim 1

A method for the prevention and/or treatment of contamination of a product or a surface by Listeria monocytogenes, the method comprising contacting the product or surface with a mixture comprising at least one peptide having sequence VRLIVKVRIWRR (SEQ ID NO: 3), or a salt or solvate thereof.

### Claim 2 (depends on 1)

The method according to claim 1, wherein said mixture further comprises at least one peptide selected from the group consisting of VRLIVAVRIWRR (SEQ ID NO: 1), VRLIVAVRIKRR (SEQ ID NO: 2), and VRLIVKVRIKRR (SEQ ID NO: 4), or a salt or solvate thereof.

### Claim 3 (depends on 1)

The method according to claim 1, wherein said mixture further comprises at least one peptide selected from the group consisting of VRLIVAVRIWRR (SEQ ID NO: 1) and VRLIVAVRIKRR (SEQ ID NO: 2) and at least one peptide having sequence VRLIVKVRIKRR (SEQ ID NO: 4), or a salt or solvate thereof.

### Claim 4 (depends on 1)

The method according to claim 1, wherein said mixture is not in association with other bactericidal compounds.

### Claim 5 (depends on 1)

The method according to claim 1, wherein said product is a food, a material, or an object.

### Claim 6 (depends on 1)

The method according to claim 1, wherein said mixture is applied as a coating composition on said product or surface or linked with a covalent bond to said product or surface.

### Claim 7 (depends on 1)

The method according to claim 1, wherein said at least one peptide having sequence VRLIVKVRIWRR (SEQ ID NO: 3) is linked to the surface of nanoparticles.

### Claim 8 (depends on 1)

The method according to claim 1, wherein said Listeria monocytogenes is an antibiotic-resistant Listeria monocytogenes serotype.

### Claim 9

A method for the treatment of an infection from Listeria monocytogenes in a subject in need thereof, said method comprising administering a mixture comprising at least one peptide having sequence VRLIVKVRIWRR (SEQ ID NO: 3) or a pharmaceutically acceptable salt or solvate thereof.

### Claim 10 (depends on 9)

The method according to claim 9, wherein said Listeria monocytogenes is an antibiotic-resistant Listeria monocytogenes serotype.

### Claim 11 (depends on 9)

The method according to claim 9, wherein said mixture further comprises at least one peptide selected from the group consisting of VRLIVAVRIWRR (SEQ ID NO: 1), VRLIVAVRIKRR (SEQ ID NO: 2) and VRLIVKVRIKRR (SEQ ID NO: 4), or a pharmaceutically acceptable salt or solvate thereof.

### Claim 12 (depends on 10)

The method according to claim 10, wherein said at least one peptide having sequence VRLIVKVRIWRR (SEQ ID NO: 3) is covalently linked to the surface of nanoparticles.