# US 12,109,176 B1 · Effect of glycerol on biofilm forming bacteria and fungi that changes the microbes sensitivity to pro and anti-biofilm non-toxic, non-bonded plasma amino acids and amino acid derivatives

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- Number: US 12,109,176 B1
- Document ID: US12109176B1
- Status: Granted
- Kind: B1
- Application number: 18/304,192
- Filed: 2023-04-20
- Granted: 2024-10-08
- Earliest claimed: 2023-04-20
- Applicants: Thomas Bryan
- Inventors: Thomas Bryan
- Web page: https://patentgrep.com/patent/US12109176B1

## CPC

- A61K 31/047: having two or more hydroxy groups, e.g. sorbitol
- A61K 31/198: Alpha-amino acids, e.g. alanine or edetic acid [EDTA] (betaine A61K31/205; proline A61K31/401; tryptophan A61K31/405; histidine A61K31/4172; peptides not degraded to individual amino acids A61K38/00)
- A61K 45/06: Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
- A61P 31/04: Antibacterial agents
- A61P 31/10: Antimycotics
- C02F 1/50: by addition or application of a germicide or by oligodynamic treatment {(C02F1/4606, C02F1/467, C02F1/76 take precedence)}
- C02F 2303/04: Disinfection
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes)

## Abstract

A method of desensitizing microbes to pro-biofilm amino acids and amino acid derivatives in plasma comprising administering a sufficient amount of glycerol. A method of sensitizing microbes to anti-biofilm amino acids and amino acid derivatives in plasma comprising administering a sufficient amount of glycerol. Methods of increasing a plasma concentration of glycerol comprising administering a sufficient amount of glycerol. A method of treating an infection and/or contamination comprising applying glycerol and anti-biofilm amino acids and/or amino acid derivatives to a surface to be treated and/or water to be treated. A method of treating a biofilm producing viral infection comprising administering a sufficient, non-toxic concentration of glycerol. A method of treating a protozoal infection comprising administering a sufficient, non-toxic concentration of glycerol. A method of treating a hybrid infection comprising administering a sufficient, non-toxic concentration of glycerol and anti-biofilm amino acids and/or amino acid derivatives.

## Claims

### Claim 1

A method of desensitizing microbes to pro-biofilm amino acids and amino acid derivatives in plasma comprising administering a solution comprising a sufficient, non-toxic concentration of glycerol, wherein glycerol is the sole therapeutic agent.

### Claim 2 (depends on 1)

The method of claim 1, wherein the step of administering comprises administering to a wound the solution.

### Claim 3 (depends on 1)

The method of claim 1, wherein the step of administering comprises administering the solution vaginally, rectally, orally, intravenously, intra-arterially, intracavernously, intrathecally, and/or topically.

### Claim 4 (depends on 1)

The method of claim 1, wherein 1.7 to 4.3 g/h of glycerol are administered intravenously and/or intra-arterially.

### Claim 5 (depends on 1)

The method of claim 1, further comprising the step of administering at least one antimicrobial.