# US 12,108,764 B2 · Growth-independent inhibition of aflatoxin biosynthesis with selected nanoparticles

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 12,108,764 B2
- Document ID: US12108764B2
- Status: Granted
- Kind: B2
- Application number: 15/911,327
- Filed: 2018-03-05
- Published: 2018-09-13
- Granted: 2024-10-08
- Earliest claimed: 2017-03-03
- Assignees: University of South Carolina
- Applicants: University of South Carolina
- Inventors: Anindya Chanda; Jamie Lead
- Web page: https://patentgrep.com/patent/US12108764B2

## CPC

- A01N 59/16: Heavy metals; Compounds thereof
- A01N 25/28: Microcapsules {or nanocapsules}
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS

## Abstract

Methods for inhibiting fungal secondary metabolisms are described. Secondary metabolisms inhibited by the methods can include those responsible for expression of mycotoxins such as aflatoxin. Methods can include inhibition of aflatoxin production without inhibiting fungal growth by contacting a fungus with a suspension including silver nanoparticles that can include a surface coating. The methods can be utilized in preventing fungal-caused destruction of plant species (e.g., crop species). Disclosed methods encompass utilization of silver nanoparticles at low concentrations of from about 10 picograms per liter to about 1 microgram per liter.

## Claims

### Claim 1

A method for inhibiting production of an aflatoxin in a fungus comprising:
forming a first suspension of silver nanoparticles, the silver nanoparticles comprising a surface coating, the coated silver nanoparticles having an average size of from 15 nanometers to 30 nanometers as determined by transmission electron microscopy;
separating the coated silver nanoparticles from the first suspension by ultrafiltration in a diafiltration mode;
redispersing the coated silver nanoparticles in a second suspension, the second suspension including the coated silver nanoparticles at a concentration of from 25 nanograms per milliliter to 60 nanograms per milliliter;
contacting the fungus with the second suspension, wherein during a period of time in which the fungus is in contact with the suspension, fungal aflatoxin production is inhibited with no significant decrease in fungal growth rate as expressed as dry weight accumulation per unit time of the contact.

### Claim 2 (depends on 1)

The method of claim 1, wherein the fungus comprises a mycotoxin-producing filamentous fungus.

### Claim 3 (depends on 1)

The method of claim 1, wherein the fungus comprises Aspergillus parasiticus.

### Claim 4 (depends on 1)

The method of claim 1, the coated silver nanoparticles having an average size of 20 nanometers.

### Claim 5 (depends on 1)

The method of claim 1, wherein the coating comprises a citrate.

### Claim 6 (depends on 1)

The method of claim 1, wherein the coating comprises a polymer.

### Claim 7 (depends on 1)

The method of claim 1, the method decreasing generation of reactive oxygen species by the fungus during the period of time of contact.

### Claim 8 (depends on 1)

The method of claim 1, the method inhibiting transcription of one or more genes associated with aflatoxin biosynthesis during the period of time of contact.

### Claim 9 (depends on 1)

The method of claim 1, the method inhibiting the global regulators of secondary metabolism laeA and veA during the period of time of contact.

### Claim 10 (depends on 1)

The method of claim 1, the second suspension including the coated silver nanoparticles at a concentration of 50 nanograms per milliliter.

### Claim 11 (depends on 6)

The method of claim 6, wherein the coating comprises polyvinyl pyrrolidone.

### Claim 12 (depends on 1)

The method of claim 1, wherein the second suspension includes the coated silver nanoparticles at a concentration of from 40 nanograms per milliliter to 60 nanograms per milliliter.

### Claim 13 (depends on 1)

The method of claim 1, wherein the second suspension includes the coated silver nanoparticles at a concentration of 25 nanograms per milliliter.