# US 12,108,729 B2 · Bean plant named HMX0175722

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- Number: US 12,108,729 B2
- Document ID: US12108729B2
- Status: Granted
- Kind: B2
- Application number: 17/587,959
- Filed: 2022-01-28
- Published: 2023-08-03
- Granted: 2024-10-08
- Earliest claimed: 2022-01-28
- Assignees: Hm.Clause, Inc.
- Applicants: Hm.Clause, Inc.
- Inventors: Robert Gehin
- Web page: https://patentgrep.com/patent/US12108729B2

## CPC

- A01H 6/545: {Phaseolus, e.g. kidney beans, scarlet runners or spotted beans}
- A01H 5/10: Seeds
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

A bean plants designated HMX0175722 is disclosed. In some embodiments, the disclosure relates to the seeds of bean plant HMX0175722, to the plants and plant parts of bean HMX0175722, and to methods for producing a bean plant by crossing the bean plant HMX0175722 with itself or another bean plant. The disclosure further relates to methods for producing other bean plants derived from the bean HMX0175722.

## Claims

### Claim 1

A seed of bean plant designated HMX0175722, wherein a representative sample of seed of said bean plant has been deposited under NCIMB No. 44220.

### Claim 2 (depends on 1)

A bean plant, a part thereof, or a cell thereof, produced by growing the seed of claim 1, wherein the bean plant has all of the physiological and morphological characteristics of bean designated HMX0175722 deposited under NCIMB No. 44220 when grown under the same environmental conditions.

### Claim 3 (depends on 2)

The bean part of claim 2, wherein the bean part is selected from the group consisting of: a leaf, a flower, a pod, a bean, an ovule, an ovary, a pericarp, a legume, a seed, a stalk, a stem, a root, a rootstock, a scion, an embryo, a stamen, an anther, a pistil, a pollen, and a cell.

### Claim 4 (depends on 2)

A tissue culture of regenerable cells produced from the plant or plant part of claim 2, wherein a plant regenerated from the tissue culture has all of the physiological and morphological characteristics of bean plant HMX0175722 deposited under NCIMB No. 44220 when grown in the same environmental conditions.

### Claim 5 (depends on 4)

A bean plant regenerated from the tissue culture of claim 4, said plant having all of the physiological and morphological characteristics of bean plant HMX0175722 deposited under NCIMB No. 44220.

### Claim 6 (depends on 2)

A bean pod or seed produced from the plant of claim 2.

### Claim 7 (depends on 2)

A method for producing a bean pod, the method comprising a) growing the bean plant of claim 2 to produce a bean pod, and b) harvesting said bean pod.

### Claim 8 (depends on 7)

A bean pod produced by the method of claim 7.

### Claim 9 (depends on 2)

A method for producing a bean seed, the method comprising (a) crossing a first parent bean plant with a second parent bean plant and (b) harvesting the resultant bean seed, wherein said first parent bean plant and/or second parent plant is the bean plant of claim 2.

### Claim 10 (depends on 9)

A bean seed produced by the method of claim 9.

### Claim 11 (depends on 2)

A method for producing a bean seed, the method comprising (a) self-pollinating the bean plant of claim 2 and (b) harvesting the resultant bean seed.

### Claim 12 (depends on 11)

A bean seed, or a plant produced by growing said seed, produced by the method of claim 11.

### Claim 13 (depends on 2)

A method of vegetatively propagating the bean plant of claim 2, the method comprising: (a) collecting a part capable of being propagated from the plant of claim 2, (b) regenerating a plant from said part, and (c) harvesting a pod or seed from said regenerated plant.

### Claim 14 (depends on 13)

A plant, pod, or seed obtained from the method of claim 13, wherein said plant has all of the physiological and morphological characteristics of bean plant HMX0175722 deposited under NCIMB No. 44220 when grown under the same environmental conditions.

### Claim 15 (depends on 2)

A method of producing a bean plant obtained from the bean plant HMX0175722, the method comprising the steps of:
(a) self-pollinating the bean plant of claim 2 or crossing the bean plant of claim 2 with a second bean plant to produce a progeny bean plant obtained from bean plant HMX0175722;
(b) self-pollinating the progeny bean plant of step (a) or crossing the plant of step (a) with a second bean plant to produce a progeny seed of a subsequent generation;
(c) growing a progeny plant from the progeny seed of the subsequent generation; and
(d) self-pollinating the progeny plant of step (c) or crossing the plant of step (c) with a second bean plant to produce a bean plant derived from the bean plant HMX0175722.

### Claim 16 (depends on 15)

The method of claim 15, further comprising the step of:
(e) repeating step (c) or (d) for at least one more generation to produce a bean plant further derived from the bean plant HMX0175722.

### Claim 17

A method of producing a plant of bean plant designated HMX0175722 comprising at least one desired trait, the method comprising: introducing a single locus conversion conferring the desired trait into bean plant designated HMX0175722 deposited under NCIMB No. 44220, whereby bean plant designated HMX0175722 comprising the desired trait is produced.

### Claim 18 (depends on 17)

A bean plant, a part thereof, or a cell thereof, produced by the method of claim 17, wherein the plant, the part, or the cell thereof comprises a single locus conversion and otherwise all of the physiological and morphological characteristics of bean plant designated HMX0175722 deposited under NCIMB No. 44220.

### Claim 19 (depends on 18)

The plant of claim 18, wherein the single locus conversion confers said plant with a trait selected from the group consisting of male sterility, male fertility, herbicide resistance, insect resistance, disease resistance, water stress tolerance, and heat tolerance.

### Claim 20 (depends on 18)

The plant of claim 18, wherein the single locus conversion is introduced into the plant by a gene-editing technique with a nuclease selected from the group consisting of Zinc finger nuclease (ZFN), Transcription Activation-Like Effector Nuclease (TALEN), Clustered Regularly Interspaced Short Palindromic repeats-associated endonuclease Cas9 (CRISPR-Cas9), engineered meganuclease, and engineered homing endonuclease.

### Claim 21

A method of introducing a desired trait into bean plant HMX0175722, the method comprising a step of:
(a) crossing a bean plant HMX0175722 plant grown from bean plant HMX0175722 seed, wherein a representative sample of seed has been deposited under NCIMB No. 44220, with another bean plant that comprises a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of insect resistance, disease resistance, water stress tolerance, and heat tolerance;
(b) selecting one or more progeny plants that have the desired trait to produce one or more selected progeny plants;
(c) crossing the one or more selected progeny plants with a plant of variety HMX0175722 to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have the desired trait and the physiological and morphological characteristics of bean plant HMX0175722 deposited under NCIMB No. 44220 when grown under the same environmental condition to produce selected backcross progeny plants; and
(e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and the physiological and morphological characteristics of bean plant HMX0175722 deposited under NCIMB No. 44220 when grown under the same environmental conditions.

### Claim 22 (depends on 21)

A bean plant produced by the method of claim 21, wherein the plant has the desired trait and otherwise all of the physiological and morphological characteristics of bean plant HMX0175722 deposited under NCIMB No. 44220 when grown under the same environmental condition.

### Claim 23 (depends on 2)

A method of producing a bean plant, the method comprising grafting a rootstock or a scion of the bean plant of claim 2 to another bean plant.

### Claim 24 (depends on 2)

A method for producing nucleic acids, the method comprising isolating nucleic acids from the plant of claim 2, a part, or a cell thereof.
