# US 12,108,726 B2 · Inbred corn line MN40

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- Number: US 12,108,726 B2
- Document ID: US12108726B2
- Status: Granted
- Kind: B2
- Application number: 17/719,018
- Filed: 2022-04-12
- Published: 2023-10-12
- Granted: 2024-10-08
- Earliest claimed: 2022-04-12
- Assignees: Limagrain Europe S.A.; KWS SAAT SE & Co. KGaA
- Applicants: KWS SAAT SE & Co. KGaA; Limagrain Europe S.A.
- Inventors: Phyll Cammack
- Web page: https://patentgrep.com/patent/US12108726B2

## CPC

- A01H 6/4684: {Zea mays [maize]}
- A01H 5/10: Seeds
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

Inbred corn line, designated MN40, are disclosed. The disclosure relates to the seeds of inbred corn line MN40, to the plants and plant parts of inbred corn line MN40 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line MN40 with itself or another corn line. The disclosure also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line MN40 and/or of the hybrids produced using the inbred as a parent. The disclosure further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other corn lines derived from inbred corn line MN40.

## Claims

### Claim 1

A seed of inbred corn line designated MN40, wherein a representative sample of seed of said line was deposited under NCMA Accession No. 202404001.

### Claim 2 (depends on 1)

A corn plant, or a part thereof, produced by growing the seed of claim 1.

### Claim 3

A corn plant, or a part thereof, having all of the physiological and morphological characteristics of inbred corn line MN40, wherein a representative sample of seed of said line was deposited under NCMA Accession No. 202404001.

### Claim 4 (depends on 2)

A tissue culture of cells produced from the plant or the part of claim 2.

### Claim 5 (depends on 4)

A corn plant regenerated from the tissue culture of claim 4, wherein the regenerated plant has all of the morphological and physiological characteristics of inbred corn line MN40, wherein a representative sample of seed of said line was deposited under NCMA Accession No. 202404001.

### Claim 6 (depends on 2)

A method for producing a hybrid corn seed, the method comprising: crossing the plant of claim 2 with a different corn plant, and harvesting the resultant hybrid corn seed.

### Claim 7 (depends on 6)

A hybrid corn seed produced by the method of claim 6.

### Claim 8 (depends on 7)

An F1 hybrid corn plant, or a part thereof, produced by growing the seed of claim 7.

### Claim 9

A method for producing inbred corn line MN40, wherein a representative sample of seed of said line was deposited under NCMA Accession No. 202404001, the method comprising:
(a) planting a collection of seeds comprising seed of a hybrid, one of whose parents is inbred corn line MN40, the collection also comprising seed of the inbred corn line;
(b) growing plants from the collection of seeds;
(c) identifying the plants having all of the physiological and morphological characteristics of inbred corn line MN40 as inbred parent plants;
(d) controlling pollination of the inbred parent plants in a manner that preserves the homozygosity of the inbred parent plants; and
(e) harvesting the resultant seed and thereby producing an inbred corn line having all of the physiological and morphological characteristics of inbred corn line MN40.

### Claim 10 (depends on 9)

The method of claim 9, wherein step (c) comprises identifying plants with decreased vigor compared to the other plants grown from the collection of seeds.

### Claim 11 (depends on 2)

A method for producing a corn plant that contains in its genetic material one or more transgenes, the method comprising: crossing the plant of claim 2 with either a second plant of another corn line that contains a transgene or a transformed corn plant of the inbred corn line MN40, so that the genetic material of a progeny plant that results from the cross contains the transgene(s) operably linked to a regulatory element, wherein the transgene confers a trait selected from the group consisting of male sterility, male fertility, herbicide resistance, insect resistance, disease resistance, water stress tolerance, and increased digestibility.

### Claim 12 (depends on 11)

A corn plant, or a part thereof, produced by the method of claim 11.

### Claim 13 (depends on 12)

The corn plant of claim 12, wherein the transgene confers resistance to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.

### Claim 14 (depends on 12)

The corn plant of claim 12, wherein the transgene encodes a Bacillus thuringiensis protein.

### Claim 15 (depends on 12)

The corn plant of claim 12, wherein the transgene confers disease resistance.

### Claim 16 (depends on 12)

The corn plant of claim 12, wherein the transgene confers water stress tolerance.

### Claim 17 (depends on 12)

The corn plant of claim 12, wherein the transgene confers increased digestibility.

### Claim 18 (depends on 12)

A method for producing a hybrid corn seed, the method comprising: crossing the plant of claim 12 with a different corn plant, and harvesting the resultant hybrid corn seed.

### Claim 19 (depends on 2)

A method of producing a corn plant with increased waxy starch or increased amylose starch, the method comprising: transforming the corn plant of claim 2 with a transgene that modifies waxy starch or amylose starch metabolism, thereby producing the corn plant with increased waxy starch or amylose starch metabolism.

### Claim 20 (depends on 19)

A corn plant produced by the method of claim 19.

### Claim 21

A method of introducing one or more desired traits into inbred corn line MN40, the method comprising:
(a) crossing inbred corn line MN40 plants grown from inbred corn line MN40 seeds, wherein a representative sample of seed of the line was deposited under NCMA Accession No. 202404001, with plants of another corn line that comprise one or more desired traits to produce progeny plants, wherein the one or more desired traits are selected from the group consisting of male sterility, male fertility, herbicide resistance, insect resistance, disease resistance, waxy starch, water stress tolerance, increased amylose starch and increased digestibility;
(b) selecting progeny plants that have the one or more desired traits to produce selected progeny plants;
(c) crossing the selected progeny plants with the inbred corn line MN40 plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have the one or more desired traits to produce selected backcross progeny plants; and
(e) repeating steps (c) and (d) one or more times in succession to produce selected second or higher backcross progeny plants that comprise the one or more desired traits and all of the physiological and morphological characteristics of inbred corn line MN40.

### Claim 22 (depends on 21)

A corn plant produced by the method of claim 21, wherein the plant has the one or more desired traits and otherwise all of the physiological and morphological characteristics of inbred corn line MN40.

### Claim 23 (depends on 2)

A method for producing inbred corn line MN40 seed, wherein a representative sample of seed of said line was deposited under NCMA Accession No. 202404001, the method comprising: crossing a first inbred corn plant with a second inbred corn plant, and harvesting the resultant corn seed, wherein both the first and second inbred corn plants are the plant of claim 2.

### Claim 24 (depends on 1)

A method for producing inbred corn line MN40 seed, wherein a representative sample of seed of said line was deposited under NCMA Accession No. 202404001, the method comprising:
(a) planting the seed of claim 1;
(b) growing a plant from said seed;
(c) controlling pollination in a manner that the pollen produced by the grown plant pollinates the ovules produced by the grown plant; and
(d) harvesting the resultant seed and thereby producing an inbred corn line having all of the physiological and morphological characteristics of inbred corn line MN40.

### Claim 25 (depends on 2)

A method for producing a corn seed that contains in its genetic material one or more transgenes, wherein the method comprises crossing the corn plant of claim 2 with either a second plant of another corn line which contains one or more transgenes or a transformed corn plant of the inbred corn line MN40, wherein the transgene(s) is operably linked to a regulatory element and wherein the transgene confers a trait selected from the group consisting of male sterility, male fertility, herbicide resistance, insect resistance, disease resistance, water stress tolerance, and increased digestibility, and harvesting the resultant seed.

### Claim 26 (depends on 25)

A corn seed, or a part thereof, produced by the method of claim 25.

### Claim 27 (depends on 22)

A method for producing a hybrid corn seed, the method comprising: crossing the plant of claim 22 with a different corn plant, and harvesting the resultant hybrid corn seed.

### Claim 28 (depends on 27)

An F1 hybrid corn seed produced by the method of claim 27.

### Claim 29 (depends on 1)

A method of producing a corn product, the method comprising the step of milling the seed of claim 1, thereby producing the corn product.

### Claim 30 (depends on 29)

The method of claim 29, wherein the corn product is selected from the group consisting of corn meal, corn flour, corn starch, corn syrup, corn sweetener and corn oil.

### Claim 31 (depends on 7)

A method of producing a corn product, the method comprising the step of milling the hybrid seed of claim 7, thereby producing the corn product.

### Claim 32 (depends on 31)

The method of claim 31, wherein the corn product is selected from the group consisting of corn meal, corn flour, corn starch, corn syrup, corn sweetener and corn oil.
