PSMA-based molecular-genetic reporter system
AssigneeThe Johns Hopkins University
InventorsMartin G. Pomper, Mark Castanares, Il Minn, Shawn Lupold
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Abstract
Methods, reporter gene constructs, and kits for using prostate-specific membrane antigen (PSMA) as an imaging reporter to image a variety of cells and tissues are provided.
Claims14
An adenoviral reporter gene construct comprising a prostate-specific membrane antigen (PSMA) gene, wherein the PSMA gene is CpG-free and operably linked to a cytomegalovirus immediate-early gene (CMV) promoter and wherein the PSMA gene is mutated such that the construct expresses a mutated PSMA protein that has glycine as a second amino acid instead of tryptophan.
A non-viral, episomal reporter gene construct comprising a prostate-specific membrane antigen (PSMA) gene, wherein the PSMA gene is CpG-free and operably linked to a PEG-Promoter and wherein the PSMA gene is mutated such that the construct expresses a mutated PSMA protein that has glycine as a second amino acid instead of tryptophan.
Dependencies are inferred from the claim text.