Systems and methods for processing nucleic acid molecules from one or more cells
Assignee10x Genomics, Inc.
InventorMichael Schnall-Levin
Life of this patent
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Abstract
The present disclosure provides methods and systems for processing nucleic acid molecules from one or more cells. A cell may be comprised in a cell bead. Processing may comprise uniquely identifying nucleic acid molecules. Nucleic acid molecules may be identified by barcoding. Barcoding may be combinatorial barcoding. Combinatorial barcoding may allow for generation of a large number of barcodes, thereby uniquely identifying nucleic acids from a large number of single cells. Combinatorial barcoding may be performed in successive operations. Successive operations may be performed in partitions.
Claims23
A method for processing nucleic acids, comprising:
- (a) in a first partition comprising a cell and a polymeric or gel precursor, using a template nucleic acid molecule from said cell and a first nucleic acid molecule attached to said polymeric or gel precursor to generate a second nucleic acid molecule comprising a sequence of the template nucleic acid molecule or a complement thereof;
- (b) subsequent to (a), subjecting said first partition to conditions sufficient to induce polymerization or gelling of said polymeric or gel precursor to generate a cell bead encapsulating said cell, wherein said second nucleic acid molecule is attached to said cell bead;
- and
- (c) combinatorially synthesizing a plurality of barcode sequences onto said second nucleic acid molecule or single-stranded derivative thereof within said cell bead, thereby generating a barcoded nucleic acid molecule, wherein said single-stranded derivative thereof comprises at least a portion of said template nucleic acid molecule of said cell.
Dependencies are inferred from the claim text.