Method for enzymatic sulfurylation of alcohols and amines using bacterium of the family Enterobacteriaceae
AssigneeAjinomoto Co., Inc.
InventorsSergey Vasilievich Smirnov, Irina Lvovna Tokmakova
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Abstract
A method for enzymatic sulfurylation of a substrate is provided which includes the steps of reacting the substrate with 3′-phosphoadenosine-5′-phosphosulfate (PAPS) in a medium containing a bacterium belonging to the family Enterobacteriaceae to produce a sulfated derivative of the substrate, and collecting the sulfated derivative from the medium, wherein the bacterium has been modified to produce, at least, a protein having sulfotransferase activity, and to attenuate expression of an aphA gene, a cysQ gene, or a cpdB gene, or a combination of these.
Claims17
A method for enzymatic sulfurylation of a substrate or for producing a sulfated derivative of a substrate comprising:
- (i) reacting the substrate with 3′-phosphoadenosine-5′-phosphosulfate in a medium containing a bacterium belonging to the genus Escherichia to produce a sulfated derivative of said substrate, and
- (ii) collecting the sulfated derivative from the medium;
- wherein said substrate has a chemical group selected from the group consisting of a hydroxyl group, an amino group, and combinations thereof;
- and
- wherein said bacterium has been modified
- (A) to produce, at least, a protein having sulfotransferase activity, and
- (B) to attenuate expression of an aphA gene or a cysQ gene.
Dependencies are inferred from the claim text.