Cofactor self-sufficient Escherichia coli and construction method and application thereof

A cofactor self-sufficient Escherichia coli, obtained by a constructing methods as follows:

• (1) constructing one or more plasmids or expression vectors capable of intracellular linearization and self-recombination that comprise target genes including a NADH kinase gene, a glucose dehydrogenase gene, a glufosinate dehydrogenase gene and a gene of a NADP cofactor synthesis pathway;
• transforming the one or more plasmids or expression vectors comprising the target genes into an Escherichia coli named E. coli BL21 (DE3);
• and
• obtaining a transformed Escherichia coli with introduction of the one or more plasmids or expression vectors comprising the target genes that is a glufosinate dehydrogenase-glucose dehydrogenase-NADH kinase co-expressing gene engineering bacterium;
• wherein said NADH kinase gene sequence is one of SEQ ID NOS:
• 1 to 3, said glucose dehydrogenase gene sequence is as shown in SEQ ID NO:
• 6, said glufosinate dehydrogenase gene sequence is as shown in SEQ ID NO:
• 7, and said NADP cofactor synthesis pathway gene sequence is as shown in one of SEQ ID NOS:
• 8 and 10;
• and
• (2) knocking out of any one or a combination of mazG and nadR genes in the genome of the co-expressing gene engineering bacterium to obtain said cofactor self-sufficient Escherichia coli.