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AssigneeAgricultural Genomics Institute At Shenzhen, Chinese Academy of Agricultural Sciences
InventorsJianbin Yan, Junbo Gou, Qinggang Liao, Haifei Liang, Chong Li, Ran Du, Xingyao Xiong, Sanwen Huang
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Provided are a taxadiene synthase TcTS2, an encoding nucleotide sequence and use thereof. The amino acid sequence of TcTS2 includes or consists of: (a) an amino acid sequence represented by SEQ ID NO: 1; or (b) a functional homologous sequence having at least 80% sequence similarity with the amino acid sequence represented by SEQ ID NO: 1; or (c) an amino acid sequence having TcTS2 activity with addition, deletion, or substitution of one or more amino acids in the amino acid sequence represented by SEQ ID NO: 1. TcTS2 and the nucleotide sequence encoding the TcTS2 provide new gene resources for improving the yield of taxol, and they may be used for modifying chassis hosts by plant genetic engineering and metabolic engineering strategies to produce taxol and the intermediates thereof etc., thereby having significant economic and social value.
A recombinant DNA vector comprising a heterologous nucleic acid sequence, wherein the nucleic acid sequence encodes a protein named TcTS2 (Taxus chinensis Taxadiene synthase 2) comprising SEQ ID NO:
A transgenic microbial cell having taxadiene synthase activity, wherein the activity is conferred by a transgene comprising a nucleic acid sequence encoding a TcTS2 protein comprising SEQ ID NO:
A transgenic plant, plant part, or plant cell having taxadiene synthase activity, wherein the activity is conferred by a transgene comprising a nucleic acid sequence encoding a TcTS2 protein comprising SEQ ID NO:
A method for producing a transgenic plant, plant part, or plant cell having taxadiene synthase activity, the method comprising:
A method for synthesis of baccatin III and/or paclitaxel, the method comprising:
A method for producing paclitaxel and an intermediate thereof, the method comprising:
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