US 12,103,980 B2 · Development of recombinant chicken IGY SCFV antibody raised against human thymidine kinase 1 expressed in prokaryotic cells and use thereof

Abstract

Disclosed are the expression of a recombinant chicken IgY scFv antibody and monoclonal antibody raised against human thymidine kinase 1 (hTK1) in prokaryotic cells and a preparation method and use thereof. The scFv antibody includes an amino acid sequence shown in SEQ ID NO: 1. The anti-hTK1 recombinant scFv antibody and monoclonal antibody have good immunoreactivity, high specificity and sensitivity, and are easy to separate and purify.

Claims12

Dependent claims

A recombinant chicken IgY scFv antibody raised against human thymidine kinase 1 and expressed in prokaryotic cells, comprising the amino acid sequence shown in SEQ ID NO:

Dependencies are inferred from the claim text.

Facts

Application

17/191,722Filed 4 Mar 2021 · continuation

Priority

CN 201811029929.15 Sep 2018

Published

US 2021/0189010 A124 Jun 2021

Granted

1 Oct 2024

Term ends

Not estimatedThe source records don’t support an estimate.

Classification15 codes

C07K 16/40
Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies: against enzymes
A61K 47/6871
The modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site: the antibody targeting an enzyme
C12N 15/70
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…
G01N 33/575
Immunoassay; Biospecific binding assay; Materials therefor: for cancer

Development of recombinant chicken IGY SCFV antibody raised against human thymidine kinase 1 expressed in prokaryotic cells and use thereof

Life of this patent

Development of recombinant chicken IGY SCFV antibody raised against human thymidine kinase 1 expressed in prokaryotic cells and use thereof

Life of this patent

Abstract

Claims12