Immunogenic sequences from a phage tail length tape measure protein, bacteria expressing the same and their use in treating a cancer
AssigneesInstitut Gustave Roussy, Institut National de la Sante et de la Recherche Medicale, Universite Paris-Saclay, Centre Hospitalier Universitaire De Rennes, Universite De Rennes
InventorLaurence Zitvogel
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Abstract
The present invention relates to the field of probiotic adjuvantization of anticancer treatments. In particular, the present invention concerns immunogenic sequences from a prophage present in bacteria identified as efficient adjuvants of cancer treatments. The invention provides bacterial compositions expressing immunogenic sequences from this prophage, immunogenic compositions comprising such sequences and methods using sequences from this prophage, for increasing the anticancer armamentarium.
Claims18
A method of treating cancer comprising administering a bacterial composition comprising at least one naturally occurring or engineered bacterial strain in combination with an antineoplastic alkylating agent, wherein said at least one bacterial strain expresses the tail tape measure protein of SEQ ID No:
- 1 or a fragment thereof comprising at least the peptides of SEQ ID Nos:
- 13 and 14, or a sequence encoding a peptide of at least 9 amino acids comprising at least one epitope selected from the group consisting of SEQ ID No:
- 55, 56, 62, 63 and 66 , with the proviso that said at least one bacterial strain is a different strain from the Enterococcus hirae strain 13144 deposited on Nov. 7, 2013 at the CNCM under the number I-4815.
A method comprising in vitro introducing, into a bacterial strain, a nucleotide sequence encoding the tail tape measure protein of SEQ ID No:
- 1 or a fragment thereof comprising at least the peptides of SEQ ID Nos:
- 13 and 14, or a sequence encoding a peptide of at least 9 amino acids comprising at least one epitope selected from the group consisting of SEQ ID No:
- 55, 56, 62, 63 and 66 and expressing said tail tape measure protein of SEQ ID No:
- 1 or fragment thereof.
A method of inducing an immune response in a human subject, comprising administering to the human subject an engineered bacterial strain that expresses the tail tape measure protein of SEQ ID No:
- 1 or a fragment thereof of at least 9 amino acids comprising at least one epitope selected from the group consisting of SEQ ID Nos:
- 13, 14, 55, 56, 62, 63 and 66, with the proviso that said at least one bacterial strain is a different strain from the Enterococcus hirae strain 13144 deposited on Nov. 7, 2013 at the CNCM under the number 1-4815.
A method of inducing an immune response in a human subject, comprising administering to the human subject an immunogenic composition comprising an isolated polypeptide comprising a sequence of at least 9 consecutive amino acids from the tail tape measure protein of SEQ ID No:
- 1 comprising at least one epitope selected from the group consisting of SEQ ID Nos:
- 13, 14, 55, 56, 62, 63 and 66, or an isolated polynucleotide encoding the same.
A method of inducing an immune response in a human subject, comprising administering to the human subject a bacteriophage composition isolated from bacteria expressing a tail tape measure protein having at least 80% identity with the tail tape measure protein of SEQ ID No:
- 1.
Dependencies are inferred from the claim text.