NANOS knock-out that ablates germline cells
AssigneesWashington State University, University of Maryland, The University Court of the University of Edinburgh
InventorsJon Michael Oatley, Christopher Bruce Alexander Whitelaw, Simon Geoffrey Lillico, Bhanu Prakash Telugu
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Abstract
The present invention provides livestock animals and methods to create recipient animals for spermatogonial stem cell transplantation through modulation of the NANOS gene. In one embodiment genome editing issued to create animals with insertions or deletions (indels) that inactivate or otherwise modulate NANOS gene activity so that resulting males lack functional germ cells yet retain functional somatic cells, and females are fertile. These males can then be transplanted with donor spermatogonial stem cells and used for breeding.
Claims10
A method of breeding porcine animals comprising:
- modifying NANOS2 gene expression in a male porcine animal by an insertion or deletion (indel) mutation to form a homozygous NANOS2 gene knock out or a bi-allele NANOS2 gene knockout, so that NANOS2 protein function is reduced or eliminated, wherein the male porcine animal produces no germline cells but retains Sertoli cell function, and wherein the modified NANOS2 gene comprises SEQ ID NO:
- 27, 28, 30, 31, 33, 34, 36, 37, 38, 40, 41, 42, 45, 46, 48, 49, 51, 56, 57, 164, 165, 166, 167, 168, 169, 172, 176, 177, 180, 181, 184, 185, 186, 190, 192, 193, 195, 199, 201, 203, or 204;
- transplanting donor spermatogonial stem cells (SSCs) to the male porcine animal so that spermatogenic colonies are generated;
- and
- introducing donor-derived sperm from the male porcine animal into a female porcine animal so that the female porcine animal becomes pregnant.
Dependencies are inferred from the claim text.