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US 12,098,415 B2Granted
Cell culturing structure including growth medium and non-growth medium
AssigneeInternational Business Machines Corporation
InventorsAmos Cahan, Guy M. Cohen, Theodore G. van Kessel, Sufi Zafar
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Abstract
A structure for culturing cells includes growth medium regions on a surface of the structure. Each of the growth medium regions includes a growth medium surface configured to receive and promote growth in a cell that is being cultured. The structure includes a non-growth medium. The non-growth medium includes a non-growth medium surface configured to receive the cell that is being cultured.
Claims13
A structure for monitoring cultured cells, the structure comprising:
- growth medium regions formed in a plurality of pores on a surface of the structure, wherein each of the growth medium regions includes a growth medium surface configured to receive and promote growth in a cell that is being cultured;
- a non-growth medium comprising the plurality of pores, the non-growth medium further comprising an electrically insulating material, wherein sidewalls of the pores are coated with a hydrophilic coating, and wherein a topmost surface of the non-growth medium is coated with a hydrophobic coating;
- a first electrode at a first location on a surface of the structure;
- a second electrode at a second location on the surface of the structure, the second electrode positioned with respect to the non-growth medium such that a conductive path does not exist between the first electrode and the second electrode;
- and
- a detector configured to detect a lowering of electrical impedance between the first electrode and the second electrode when a conductive colony of cells provides the conductive path across the non-growth medium.
3 more elements
Dependencies are inferred from the claim text.