Method for removing dissolved oxygen, used in an electrochemical biosensor that uses an oxidase as the sensor enzyme
AssigneeRuhr-Univeristat Bochum
InventorsNicolas Plumeré, Huijie Frau Zhang, Adrian Ruff, Tobias Vöpel
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Abstract
The present invention relates to a method for removing oxygen, used in an electrochemical biosensor, and to a measurement system and a method for electrochemically determining a concentration of an analyte using said method.
Claims20
A method for removing dissolved oxygen in an electrochemical biosensor which uses a first oxidase for oxygen removal and uses a second oxidase as sensor enzyme, wherein
- i) in a first step, a first reaction is catalyzed by the first oxidase for oxygen removal, the first reaction involving reaction of oxygen with a reducing agent to form hydrogen peroxide without electrons being transferred to an acceptor other than oxygen, wherein the only component of the electrochemical biosensor oxidized in the first reaction is the reducing agent,
- ii) in a second step, the hydrogen peroxide is disproportionated to form water in a second reaction,
- iii) in a third step, the second oxidase serving as sensor enzyme catalyzes the oxidation of an analyte of an aqueous solution of the electrochemical biosensor in a third reaction, the oxidase serving as sensor enzyme being specific for the analyte, the electrons released by the oxidation of the analyte being transferred solely to an electron mediator;
- optionally, wherein the only component of the aqueous solution oxidized in the third step is the analyte.
A measurement system for electrochemical determination of an analyte in an aqueous solution in an electrochemical measurement cell, the measurement cell comprising electrodes, the measurement cell comprising a first oxidase for oxygen removal and a second oxidase for sensing the analyte;
- wherein the first oxidase is capable of catalyzing oxygen removal in a first reaction involving reaction of the oxygen with a reducing agent to form hydrogen peroxide, wherein the only component of the electrochemical biosensor that is oxidized in the first reaction step is the reducing agent;
- wherein the measurement cell comprises a reaction component for disproportionation of the hydrogen peroxide in a second reaction;
- wherein the second oxidase is capable of catalyzing the oxidation of the analyte in an aqueous solution in a third reaction, wherein the second oxidase is specific for the analyte;
- wherein the measurement cell includes an electron mediator, wherein electrons released by the oxidation of the analyte are transferred solely to the electron mediator.
Dependencies are inferred from the claim text.