Recombinant Zymomonas mobilis for producing ethylene glycol, method and uses thereof
AssigneeHubei University
InventorsShihui Yang, Xiongying Yan, Mian Li, Xia Wang, Qiaoning He
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Abstract
Recombinant Zymomonas mobilis for producing ethylene glycol, method and uses thereof are provided. The recombinant Zymomonas mobilis carries and expresses genes related to a synthesis pathway of xylonic acid and genes related to a synthesis pathway of ethylene glycol.
Claims3
A recombinant Zymomonas mobilis strain for producing ethylene glycol, wherein the recombinant strain is a Zymomonas mobilis transformed with a recombinant plasmid, wherein the recombinant plasmid comprises a xdh gene, a yagF gene, a yagE gene and a ZMO1771 gene, wherein the nucleotide sequence of the xdh gene is the nucleotide sequence set forth in SEQ ID NO:
- 1, the nucleotide sequence of the yagF gene is the nucleotide sequence set forth in SEQ ID NO:
- 9, the nucleotide sequence of the yagE gene is the nucleotide sequence set forth in SEQ ID NO:
- 10, and the nucleotide sequence of the ZMO1771 gene is the nucleotide sequence set forth in SEQ ID NO:
- 15.
A method of preparing a recombinant Zymomonas mobilis strain for producing ethylene glycol comprising:
- preparing a recombinant plasmid, wherein the recombinant plasmid comprises a xdh gene, a yagF gene, a yagE gene and a ZMO1771 gene, wherein the nucleotide sequence of the xdh gene is the nucleotide sequence set forth in SEQ ID NO:
- 1, the nucleotide sequence of the yagF gene is the nucleotide sequence set forth in SEQ ID NO:
- 9, the nucleotide sequence of the yadE gene is the nucleotide sequence set forth in SEQ ID NO:
- 10, and the nucleotide sequence of the ZMO1771 gene is the nucleotide sequence set forth in SEQ ID NO:
- 15, wherein the xdh gene is expressed under the control of a Ppdc promoter, wherein the yagF gene, the yagE gene, and the ZMO1771 gene are linked by a ribosome binding sequence and expressed under the control of a Peno promoter;
- and
- transforming a Zymomonas mobilis with the recombinant plasmid, thereby preparing a recombinant Zymomonas mobilis strain for producing ethylene glycol.
Dependencies are inferred from the claim text.