Methods for decoupling yield and productivity of a non-catabolic compound produced by a host cell
AssigneeAmyris, Inc.
InventorsPenelope R. Chua, Joshua Adam Lerman, Thomas Jon Scherbart, Chandresh Thakker, Annie Ening Tsong, Hanxiao Jiang
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Abstract
Provided herein are compositions and methods for uncoupling the yield and productivity of an isoprenoid compound produced in a host cell. In some embodiments, the yield and productivity are uncoupled by genetically modifying the host cell to reduce flux through the citric acid cycle (TCA). In other embodiments, the yield and productivity are uncoupled by reducing the levels of ATP in the host cell.
Claims17
A method of decoupling yield and productivity of an isoprenoid produced in a yeast cell capable of making the isoprenoid, wherein the method comprises:
- adding one or more ATP depleting agents to the yeast cell and reducing ATP levels during production of the isoprenoid.
Dependencies are inferred from the claim text.