Metabolically engineered organisms for the production of added value bio-products
AssigneeInbiose N.V.
InventorsJo Maertens, Joeri Beauprez, Marjan De Mey
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Abstract
The present invention relates to genetically engineered organisms, especially microorganisms such as bacteria and yeasts, for the production of added value bio-products such as specialty saccharide, activated saccharide, nucleoside, glycoside, glycolipid or glycoprotein. More specifically, the present invention relates to host cells that are metabolically engineered so that they can produce said valuable specialty products in large quantities and at a high rate by bypassing classical technical problems that occur in biocatalytical or fermentative production processes.
Claims17
A metabolically engineered bacterium or yeast for production of fructose-6-phosphate or GDP-fucose, characterized in that said bacterium or yeast:
- a) has been genetically modified by introducing a heterologous gene encoding a carbohydrate hydrolase capable of splitting sucrose into glucose and fructose;
- b) comprises a fructokinase to catalyze conversion of fructose to fructose-6-phosphate;
- and
- c) has been further genetically modified to prevent loss of fructose-6-phosphate via glycolysis due to genetic disruption of an endogenous gene encoding a phosphofructokinase, a phosphoglucose isomerase, or a combination thereof
- and wherein said bacterium or yeast comprises:
- (i) a mannose-6-phosphate isomerase to catalyze conversion of fructose-6-phosphate to mannose-6-phosphate,
- (ii) a phosphomannomutase to catalyze conversion of mannose-6-phosphate to mannose-1-phosphate;
- (iii) a mannose-1-phosphate guanylyltransferase to catalyze conversion of mannose-1-phosphate to GDP-mannose;
- (iv) a GDP mannose dehydratase to catalyze conversion of GDP-mannose to GDP-4-dehydro-6-deoxy-mannose;
- and
- (v) a GDP fucose synthase to catalyze conversion of GDP-4-dehydro-6-deoxy-mannose to GDP-fucose.
Dependencies are inferred from the claim text.