Recombinant influenza viruses and constructs and uses thereof
AssigneeUniversity of Marylnd, College Park
InventorsDaniel R. Perez, Hongjun Chen, Yibin Cai, Lindomar Jose Pena, Matthew Angel
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Abstract
Disclosed are compositions and methods comprising one or more recombinant influenza viruses. Recombinant influenza viruses with mutated polymerases and/or rearranged genomes are disclosed. Constructs comprising different influenza nucleic acid sequences are also provided. Methods of inducing protecting immunity with the recombinant influenza viruses are disclosed. Also disclosed are methods of plasmid-free production of influenza virus comprising amplicons comprising one or more of influenza genes.
Claims10
A method for producing an influenza virus, comprising:
- administering to a cell:
- a) a first plasmid comprising a nucleic acid sequence comprising a rearranged influenza type A viral genome segment 2, wherein the rearranged influenza type A viral genome segment 2 comprises:
- i) a first influenza type A viral genome segment, wherein the first influenza type A viral genome segment is influenza type A viral genome segment 2 which comprises a nucleic acid sequence that encodes PB1, and
- ii) a portion of a second influenza type A viral genome segment, wherein the portion of the second influenza type A viral genome segment comprises the portion of influenza type A viral genome segment 8 comprising a nucleic acid sequence that encodes the influenza type A viral protein, NS2, wherein the NS2 nucleic acid sequence is downstream of the nucleic acid sequence that encodes PB1, and
- b) one or more second plasmids comprising remaining viral genes to produce the influenza virus;
- and culturing the cell under conditions for viral replication.
Dependencies are inferred from the claim text.