Hydrogel-enzyme systems and methods

A hydrogel-enzyme construct for performing high temperature enzymatic reaction on an organophosphate reactant, the hydrogel-enzyme construct comprising:

• a hydrogel having multiple layers of a poly(methacrylic acid) (PMAA) hydrogel-polymer;
• and
• a plurality of phosphotriesterase molecules,
• wherein adjacent hydrogel polymer layers are connected via cross-linking, individual phosphotriesterase molecules are embedded between adjacent hydrogel polymer layers, individual phosphotriesterase molecules are covalently bonded with respective individual hydrogel polymer layers, and the hydrogel-enzyme construct is capable of performing enzymatic reaction on the organophosphate reactant after the hydrogel-enzyme construct is exposed to a high temperature condition of up to 550° C., the enzymatic reaction on the organophosphate reactant by individual phosphotriesterase molecules embedded within the hydrogel occurring at a residual activity of between 20% and 100%,
• wherein the plurality of phosphotriesterase molecules comprise Protein Repair One Stop Shop PROSS) stabilized PTE S5 from Pseudomonas diminuta S5 variant (dPTE2), and the organophosphate reactant is paraoxon.

A hydrogel-enzyme construct for performing high temperature enzymatic reaction on an organophosphate reactant, the hydrogel-enzyme construct comprising:

• a silicon wafer;
• a layer of poly(glycidyl methacrylate) (PGMA) in contact with a surface of the silicon wafer;
• a hydrogel having multiple layers of a poly(methacrylic acid) (PMAA) polymer in contact with the layer of PGMA;
• and
• a plurality of phosphotriesterase molecules,
• wherein adjacent hydrogel polymer layers are connected via cross-linking,
• individual phosphotriesterase molecules are embedded between adjacent hydrogel polymer layers,
• individual phosphotriesterase molecules are covalently bonded with respective individual hydrogel polymer layers, and
• the hydrogel-enzyme construct is capable of performing enzymatic reaction on the organophosphate reactant after the hydrogel-enzyme construct is exposed to a high temperature condition of up to 550° C., the enzymatic reaction on the organophosphate reactant by individual enzyme molecules embedded within the hydrogel occurring at a residual activity of between 20% and 100%,
• wherein the plurality of phosphotriesterase molecules comprise Protein Repair One Stop Shop (PROSS) stabilized PTE S5 from Pseudomonas diminuta S5 variant (dPTE2), and the organophosphate reactant is paraoxon.