Mixing and microfluidic apparatuses related thereto

A method comprising:

• communicating a first fluid into a mixing chamber within a microfluidic device via a first fluidic input within the microfluidic device, the first fluid comprising therapeutic mRNA;
• communicating a second fluid into the mixing chamber via a second fluidic input within the microfluidic device, the second fluid comprising delivery vehicle molecules;
• mixing the first fluid with the second fluid in the mixing chamber;
• and
• communicating a third fluid from the mixing chamber via an outlet channel within the microfluidic device, the third fluid comprising a mixture of the first fluid with the second fluid.

A method comprising:

• communicating therapeutic mRNA via a first fluid through a microfluidic device;
• communicating a delivery vehicle molecules via a second fluid through the microfluidic device;
• and
• mixing the first fluid with the second fluid in the microfluidic device, the act of mixing the first fluid with the second fluid resulting in encapsulation of the therapeutic mRNA in the delivery vehicle molecules.