Microfluidic system

A component for manipulating droplets, the component comprising:

• a first microfluidic channel configured to flow droplets of a sample fluid in a first immiscible carrier fluid, wherein each droplet is separated from an immediately adjacent droplet by the first immiscible carrier fluid, wherein the first microfluidic channel comprises the droplets of the sample fluid in the first immiscible carrier fluid, wherein each droplet is separated from the immediately adjacent droplet by the first immiscible carrier fluid;
• a region comprising one or more side branch channels in fluidic communication with the first microfluidic channel, wherein at least one of the one or more side branch channels is configured to manipulate a spacing between a pair of immediately adjacent droplets by flowing a second immiscible carrier fluid between the pair of immediately adjacent droplets;
• and
• a second microfluidic channel in fluidic communication with the first microfluidic channel and the region, wherein the second microfluidic channel is configured to flow droplets having manipulated spacing between the pair of immediately adjacent droplets, and the second microfluidic channel comprises droplets having the manipulated spacing between the pair of immediately adjacent droplets.