System and method for using a single-serve nutrient spore composition for small scale farm applications

A method of incubating bacteria spores at a point-of-use comprising the following steps:

• providing a nutrient spore composition in powdered form, the nutrient spore composition comprising one or more Bacillus species in spore form;
• heating water to a temperature in a range of 60° C. to 75° C. to produce a heated water;
• streaming or dripping the heated water over the nutrient spore composition without using pressurized flow of the heated water to form a hydrated bacteria solution;
• incubating the hydrated bacteria solution for an incubation period of around 2 to 60 minutes to form a single dose of an incubated bacteria solution;
• adding the single dose to the point-of-use and wherein the point-of-use is animal drinking water, an aquaculture pond, or soil around a plant;
• and
• repeating the providing, heating, streaming or dripping, incubating, and adding steps to form a desired number of additional single doses of the incubated bacteria solution, wherein each additional single dose is added to the point-of-use once every 12 to 24 hours.

A method of incubating bacteria spores at a point-of-use comprising the following steps:

• providing a nutrient spore composition in powdered form, the nutrient spore composition comprising one or more Bacillus species in spore form;
• heating water to a temperature in a range of around 60° C. to 80° C. to produce a heated water;
• streaming or dripping the heated water over the nutrient spore composition without using pressurized flow of the heated water to form a hydrated bacteria solution;
• incubating the hydrated bacteria solution for an incubation period of around 2 to 60 minutes to form a single dose of an incubated bacteria solution;
• suspending germination of the one or more Bacillus species at an activated, metastable state by adding the incubated bacteria solution to animal drinking water having a temperature in a range of 15° C. to 32° C.