US 12,097,221 B2Granted

Platelet lysate, and method of preparation thereof

AssigneeRegrow Biosciences Private Limited

InventorsSatyen Sanghavi, Vinayak Kedage

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Abstract

The present disclosure discloses a method for preparing a lysate, from a combination of umbilical cord blood (UCB) and maternal blood (MB) derived platelets by obtaining platelet-rich plasma via sedimentation processes and then mixing platelet-rich plasmas so obtained. The present disclosure further discloses a combination of umbilical cord blood and maternal blood platelet lysate (UCB+MB PL), and the PL so obtained shows greater efficacy as cell and tissue culture media supplement over and above its individual components, umbilical cord blood platelet lysate (UCB PL) and maternal blood platelet lysate (MB PL) and especially over foetal bovine serum (FBS). Moreover, the method of the present disclosure provides an economic, environment friendly, ethical and efficacious method to reach a cell and tissue culture supplement with far-reaching therapeutic applications.

Claims18

Dependent claims

A method for preparing a platelet lysate, said method comprising:

a) obtaining an umbilical cord blood (UCB) sample;
b) contacting the umbilical cord blood (UCB) sample with at least one sedimentation reagent to obtain an umbilical cord blood (UCB) sedimentation mixture;
c) subjecting the umbilical cord blood (UCB) sedimentation mixture to at least one sedimentation to obtain an umbilical cord blood (UCB) derived platelet-rich plasma;
d) obtaining a maternal blood (MB) sample, and subjecting the maternal blood (MB) sample to at least one sedimentation to collect maternal blood (MB) derived platelet-rich plasma;
e) mixing the umbilical cord blood (UCB) derived platelet-rich plasma of step (c) and the maternal blood (MB) derived platelet-rich plasma of step (d) to obtain an umbilical cord blood and maternal blood (UCB+MB) platelet-rich plasma mixture;
f) storing the umbilical cord blood and maternal blood (UCB+MB) platelet-rich plasma mixture to obtain a frozen umbilical cord blood and maternal blood (UCB+MB) platelet-rich plasma mixture;
and
g) subjecting the frozen umbilical cord blood and maternal blood (UCB+MB) platelet-rich plasma mixture to freeze-thaw cycles to obtain a lysate from a combination of umbilical cord blood derived platelets and maternal blood derived platelets.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application: 17/428,590Filed 7 Feb 2020
Priority: IN 2019210051508 Feb 2019
Published: US 2022/0184132 A116 Jun 2022
Granted: 24 Sep 2024
Term ends: Not estimatedThe source records don’t support an estimate.

Classification9 codes

A61K 35/19
Blood; Artificial blood: platelets; Megacaryocytes
A61K 35/32
Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically…:…
A61K 35/51
Reproductive organs: umbilical cord; Umbilical cord blood; Umbilical stem cells
C12N 5/0644
Cells from the blood or the immune system: platelets; Megakaryocytes