# US 12,092,638 B2 · Biosensor using magnetic nanoparticles, and detection device and detection method using same

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- Number: US 12,092,638 B2
- Document ID: US12092638B2
- Status: Granted
- Kind: B2
- Application number: 17/305,935
- Filed: 2021-07-16
- Published: 2021-11-04
- Granted: 2024-09-17
- Earliest claimed: 2018-08-09
- Assignees: Bbb Inc.
- Applicants: Bbb Inc.
- Inventors: Hyundoo Hwang; Jaekyu Choi
- Web page: https://patentgrep.com/patent/US12092638B2

## CPC

- G01N 33/54333: {Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction}
- G01N 27/3277: {being a redox reaction, e.g. detection by cyclic voltammetry (voltammetry per se G01N27/42, G01N27/48)}
- G01N 27/3278: {involving nanosized elements, e.g. nanogaps or nanoparticles (nanopores G01N33/48721; magnetic beads G01N27/745)}
- G01N 33/54346: {Nanoparticles}
- G01N 33/5438: {Electrodes}
- G01N 33/54393: {Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding}
- G01N 33/553: Metal or metal coated
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A biosensor includes a reaction part including a magnetic nanoparticle complex, a first electrode, and a second electrode; and a sample introduction part forming a passage so that a sample can be introduced into the reaction part from an outside of the biosensor. The magnetic nanoparticle complex includes a first capturing substance for capturing a first target substance, a magnetic nanoparticle, and a reaction substance that performs at least one of an oxidation reaction and a reduction reaction.

## Claims

### Claim 1

A biosensor for detecting a target antigen, the biosensor comprising:
a contact part;
a sample introduction part;
a reaction part comprising a first electrode and a second electrode, wherein a first antibody is positioned on the first electrode, and the first electrode is configured to electrically connect with an electrode of a detection device; and
a magnetic nanoparticle complex comprising a second antibody, a reaction substance and a magnetic nanoparticle, wherein the magnetic nanoparticle has a magnetic property, and the second antibody and the reaction substance are positioned on the magnetic nanoparticle,
wherein the sample introduction part is configured to make fluidic connect with the reaction part such that a sample introduced through the sample introduction part moves to the reaction part,
wherein the first antibody positioned on the first electrode specifically binds to the target antigen,
wherein the second antibody of the magnetic nanoparticle complex specifically binds to the target antigen,
wherein the reaction substance is configured to perform an oxidation reaction or a reduction reaction near the first electrode when a predetermined condition is satisfied,
wherein the second electrode is physically separated from the first electrode,
wherein the predetermined condition is formed by a voltage applied between the first electrode and the second electrode,
wherein the reaction substance is fixed to the magnetic nanoparticle and the second antibody is fixed to the reaction substance,
wherein the second antibody is exposed to an outside of the reaction substance,
wherein the reaction substance has a property that is configured to induce a change of a current according to the voltage applied between the first electrode and the second electrode when the reaction substance is near the first electrode, to represent the target antigen is included in the sample,
wherein the contact part comprises a fourth electrode electrically connected to the first electrode and a fifth electrode electrically connected to the second electrode,
wherein at least a portion of the fourth electrode and at least a portion of the fifth electrode are exposed to an outside of the biosensor, and are electrically connected to the detection device,
wherein the detection device is configured to control the voltage applied between the first electrode and the second electrode,
wherein the detection device is programmed to perform a detection step for detection whether the target antigen is captured by detecting the current in response to a change of the applied voltage, and to perform a stabilization step for stabilizing a curve of the current,
wherein the detection step is performed after the stabilization step,
wherein the stabilization step comprises at least one of a first step of applying a first voltage between the first electrode and the second electrode for a predetermined time or longer, a second step of applying a falling voltage between the first electrode and the second electrode, or a third step of applying a raising voltage between the first electrode and the second electrode,
wherein the detection step comprises a fourth step of applying a raising voltage between the first electrode and the second electrode, or a fifth step of applying a falling voltage between the first electrode and the second electrode,
wherein the first voltage is applied as a voltage higher than a minimum voltage of at least one of a lowest voltage in the fourth step and a lowest voltage in the fifth step, and
wherein the first step is a step for performing an oxidation pretreatment of the reaction substance.

### Claim 2 (depends on 1)

The biosensor of claim 1,
wherein the reaction substance is a metal particle.

### Claim 3 (depends on 2)

The biosensor of claim 2,
wherein the reaction substance is a gold particle or a silver particle.

### Claim 4 (depends on 1)

The biosensor of claim 1,
wherein the reaction part further comprises a third electrode, the third electrode is separated from the first electrode,
wherein a position of the magnetic nanoparticle complex is controlled by an electrical field of the reaction part, and
wherein the electrical field is formed by the voltage applied between the first electrode and the third electrode.

### Claim 5 (depends on 1)

The biosensor of claim 1,
wherein the reaction part further comprises a third electrode, the third electrode is separated from the first electrode,
wherein a position of the magnetic nanoparticle complex is controlled by a magnetic field of the reaction part, and
wherein the magnetic field is formed by a current voltage applied on the third electrode.

### Claim 6 (depends on 1)

The biosensor of claim 1,
wherein the first antibody is same substance with the second antibody.

### Claim 7 (depends on 1)

The biosensor of claim 1,
wherein when the target antigen is included in the sample, the second antibody of the magnetic nanoparticle complex is immovable to the first antibody positioned on the first electrode, and
wherein when the second antibody is immovable to the first electrode indirectly, the reaction substance induces the change of a current in response to an electrical condition of the reaction part.