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US 12,092,636 B2Granted
Sensor for liquid biopsy and its method of making, and method of non-invasive liquid biopsy
AssigneeCity University of Hong Kong
InventorsYoungjin Lee, Abhimanyu Thakur, Chi-Man Lawrence Wu, Chen Xu, Siu-Pang Ng
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Abstract
A sensor for liquid biopsy, its method of making, and its method of non-invasive use. The sensor includes a substrate with a surface functionalized with biotinylated antibodies. The biotinylated antibodies are arranged to engage with surface proteins on exosomes associated with malignant cancer cells such as glioma cells.
Claims33
A sensor for liquid biopsy, comprising:
- a substrate with a surface functionalized with a plurality of first biotinylated antibodies, the surface comprising nano-holes, the first biotinylated antibodies being arranged to engage with first surface proteins on exosomes associated with malignant cancer cells with a first adhesion force capable of capturing the exosomes, wherein the nano-holes are arranged within the surface in a non-periodic manner;
- and
- a cantilever for atomic force microscopy, the cantilever being arranged to be operably cooperating with the substrate, the cantilever including a tip with a tip surface functionalized with a plurality of second biotinylated antibodies being arranged to engage with second surface proteins on the exosomes with a second adhesion force capable of sensing and quantifying the second surface proteins, wherein the second surface proteins are different than the first surface proteins.
A method of non-invasive liquid biopsy, the method comprising
- providing a solution containing exosomes associated with malignant cancer cells to a sensor comprising
- a substrate with a surface functionalized with a plurality of first biotinylated antibodies, the surface comprising nano-holes, the first biotinylated antibodies being arranged to engage with first surface proteins on the exosomes associated with malignant cancer cells with a first adhesion force capable of capturing the exosomes, wherein the nano-holes are arranged within the surface in a non-periodic manner, and
- a cantilever for atomic force microscopy, the cantilever being arranged to be operably cooperating with the substrate, the cantilever including a tip with a tip surface functionalized with a plurality of second biotinylated antibodies being arranged to engage with second surface proteins on the exosomes with a second adhesion force capable of sensing and quantifying the second surface proteins, wherein the second surface proteins are different than the first surface proteins;
- and
- detecting exosomes engaged with the surface of the substrate.
2 more elements
A method for making a sensor for liquid biopsy, the method comprising
- providing a substrate with a surface;
- functionalizing the surface with a plurality of first biotinylated antibodies, the surface comprising nano-holes, the first biotinylated antibodies being arranged to engage with first surface proteins on exosomes associated with malignant cancer cells with a first adhesion force capable of capturing the exosomes, wherein the nano-holes are arranged within the surface in a non-periodic manner;
- providing a cantilever for atomic force microscopy, the cantilever being arranged to be operably cooperating with the substrate, the cantilever including a tip with a tip surface;
- and
- functionalizing the tip surface with a plurality of second biotinylated antibodies being arranged to engage with second surface proteins on the exosomes with a second adhesion force capable of sensing and quantifying the second surface proteins, wherein the second surface proteins are different than the first surface proteins.
2 more elements
Dependencies are inferred from the claim text.