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US 12,091,697 B2Granted
Enzymatic preparation of glucosamine
AssigneeTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
InventorsChun You, Dongdong Meng
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Abstract
A method for preparing glucosamine includes the steps of converting fructose-6-phosphate (F6P) and an ammonium salt to glucosamine-6-phosphate (GlcN6P) under the catalysis of glucosamine-6-phosphate deaminase (EC 3.5.99.6, GlmD); and producing glucosamine (GlcN) by the dephosphorylation of GlcN6P under the catalysis of an enzyme capable of catalyzing the dephosphorylation. Such a method can be used to prepare glucosamine by in vitro enzymatic biosystem.
Claims22
A method for one-pot synthesis of glucosamine in vitro, comprising:
- forming a reaction mixture comprising fructose-6-phosphate (F6P), an ammonium salt, a glucosamine-6-phosphate deaminase (EC 3.5.99.6, GlmD), and a first enzyme in a reaction vessel;
- converting F6P and the ammonium salt to glucosamine-6-phosphate (GlcN6P) in the presence of the glucosamine-6-phosphate deaminase in the reaction vessel;
- and
- dephosphorylating GlcN6P in the presence of the first enzyme to produce glucosamine (GlcN) in the reaction vessel.
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Dependencies are inferred from the claim text.