Storage method and banking system of NT cell

A method of generating immunocompatible nuclear transfer (NT) cell-derived stem cells, the method comprising:

• a) obtaining HLA-A, HLA-B and HLA-DRB1 haplotype homozygous somatic cells by:
• a1) screening for human leukocyte antigen HLA-A, HLA-B, and HLA-DRB1 homozygosity in a plurality of donor tissues, and
• a2) isolating HLA-A, HLA-B, and HLA-DRB1 haplotype homozygous cells from the plurality of donor tissues;
• b) generating NT cells by:
• b0) isolating nuclei from the HLA-A, HLA-B, and HLA-DRBI haplotype homozygous somatic cells;
• b1) enucleating oocytes, wherein the enucleation is performed in a medium comprising a protein phosphatase inhibitor;
• b2) fusing the isolated nuclei of the HLA-A, HLA-B, and HLA-DRBI haplotype homozygous somatic cells to the enucleated oocytes, thereby creating NT cells, wherein the fusing is performed in a medium containing Sendai virus or a Sendai virus extract;
• b3) activating the NT cells of b2) by electrical pulses;
• b4) providing post-activating NT cells by incubating the NT cells of b3) in a post-activation medium, wherein the post-activation medium comprises 6-dimethylaminopurine (6-DMAP), trichostatin A (TSA) and an epigenetic modifying agent that is involved in modification of a lysine demethylase (KDM) domain protein, and wherein the epigenetic modifying agent contacts the nuclei of at least one of the NT cells of b3);
• and
• c) generating the stem cells from the post-activating NT cells by:
• c1) generating blastocysts from the post-activating NT cells;
• c2) isolating inner cell mass (ICM) cells from the generated blastocysts;
• and
• c3) further culturing the isolated ICM cells into the stem cells.