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US 12,091,678 B2Granted
Haploid human embryonic stem cell lines and somatic cell lines and methods of making the same
AssigneesNew York Stem Cell Foundation, Inc., Yissum Research Development Company of the Hebrew University of Jerusalem
InventorsDietrich M. Egli, Nissim Benvenisty, Ido Sagi
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Abstract
Haploid human embryonic stem cells and cell lines, haploid multipotent human cells, and haploid differentiated human cells are provided. In addition, methods of making and using the haploid human cells are provided.
Claims17
A method of genetic screening comprising:
- a) exposing an enriched population of human haploid embryonic stem (ES) cells capable of differentiating into a terminally differentiated haploid cell, to a mutagen to induce at least one mutation in the cells, wherein said terminally differentiated haploid cell is a haploid mature neuron or a haploid cardiomyocyte;
- b) selecting human haploid ES cells in said enriched population that contain said mutation;
- and
- c) identifying a genotypic and/or phenotypic effect of said mutation in said selected human haploid ES cells.
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