Method for preparing non-human primate somatic cell cloned animal

A method for preparing a non-human primate somatic cell cloned animal, comprising the steps:

• (i) providing a reconstructed oocyte, which is from the non-human primate, wherein the reconstructed oocyte is at a one-cell stage;
• (ii) performing an activation treatment on the reconstructed oocyte to obtain an activated reconstructed oocyte;
• (iii) performing a reprogramming treatment to for (a) the activated reconstructed oocyte to obtain a reprogrammed reconstructed embryo;
• and
• (iv) regenerating the reprogrammed reconstructed embryo to obtain a non-human primate somatic cell cloned animal,
• wherein:
• in step (ii), the activation treatment is performed with an electrical stimulation and an activation treatment agent comprising the combination of a calcium ion activator, a histone deacetylase inhibitor, dimethylaminopyridine (6-DMAP), and puromycin;
• in step (iii), the reprogramming treatment is performed with a reprogramming activator comprising an mRNA encoding a Kdm4d protein, wherein the mRNA is injected to the activated reconstructed oocyte at 5-6 hours post activation;
• and
• in step (iv), the “regenerating” is performed in a uterus of a non-human primate surrogate animal.