Alcohol dehydrogenase 2 (ADH2) promoter variants by promoter engineering
AssigneeOrta Dogu Teknik Universitesi
InventorsPinar Calik, Burcu Gunduz Ergun
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Abstract
Pichia pastoris alcohol dehydrogenase 2 (ADH2) promoter variants include at least one of the specified modifications on wild-type Pichia pastoris ADH2 promoter (SEQ ID NO: 1). The modification includes one of the following mutations: integration of a Cat8 transcription factor binding site (TFBS), particularly integration of SEQ ID NO: 3 or other gene sequences that show at least 80% similarity with this sequence, at any positions within nucleotides a) 647 to 660; b) 739 to 752; c) 1 to 948; and d) mutations specified with SEQ ID NO: 2 within nucleotides 15 to 848 separately and combinations thereof.
Claims14
A Pichia pastoris alcohol dehydrogenase 2 (ADH2) promoter variant, comprising at least one mutation, wherein the at least one mutation is from integration of a Cat8 transcription factor binding site (TFBS) at any positions within nucleotides 647 to 660;
- or 739 to 752 of SEQ ID NO:
- 1, wherein the Cat8 TFBS is set forth by SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.