Partition-based method of analysis
AssigneeBio-Rad Laboratories, Inc.
InventorsDonald A. Masquelier, Kevin D. Ness, Benjamin J. Hindson, Billy W. Colston, Jr.
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Abstract
Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.
Claims18
A method for quality control in digital PCR, the method comprising the steps of:
- preparing a reaction mixture comprising a target nucleic acid, a first dye, a second dye, and nucleic acid amplification reagents;
- generating a plurality of partitions comprising the reaction mixture;
- and
- assessing the first dye or and the second dye to indicate variation among the partitions, the first dye providing a test signal and the second dye providing a reference signal and indicating a partition volume of the partitions.
A method for quality control in a digital PCR, the method comprising:
- distributing a reaction mixture comprising a target nucleic acid, a first dye, a second dye, and nucleic acid amplification reagents into a plurality of partitions;
- and
- assessing the first dye or and the second dye to indicate presences of the reaction mixture within members of the plurality, the first dye providing a test signal and the second dye providing a reference signal and indicating a partition volume of the partitions.
Dependencies are inferred from the claim text.