Composite tubular material prepared from extracellular matrix and synthetic polymer, and preparation method thereof

A preparation method of a composite tubular material from an extracellular matrix and synthetic polymer fibers, comprising the following steps:

• step 1:
• preparing uniform extracellular matrix solution, comprising steps of selecting animal source tissue, removing external fat, connective tissue and blood, thus yielding a first process source tissue, and cutting the first process source tissue into segments or pieces;
• sterilizing and disinfecting the segments or pieces to obtain sterilized and disinfected segments or pieces, repeatedly freezing and thawing the sterilized and disinfected segments or pieces to yield a second process source tissue, destructing cell membrane components of the second process source tissue through elution with polyethylene glycol mono(4-tert-octylphenyl) ether, sodium deoxycholate or sodium dodecyl sulfate, then removing nucleic acid components by eluting with DNase and RNase to yield a third process source tissue;
• freeze drying the third process source tissue to obtain freeze dried tissue and grinding the freeze dried tissue into powder, and dissolving the powder in acid and stirring to obtain the uniform extracellular matrix solution with a mass/volume fraction ranging from 0.1% to 95%;
• step 2:
• constructing of a synthetic polymer fiber skeleton by processing a synthetic polymer through melt spinning or wet spinning;
• and
• step 3:
• preparing the composite tubular material from the uniform extracellular matrix solution and the synthetic polymer fiber skeleton through compounding, comprising soaking the synthetic polymer fiber skeleton in the uniform extracellular matrix solution to obtain a combination of the synthetic polymer fiber skeleton and the uniform extracellular matrix solution;
• freeze-drying or repeatedly air-drying the combination to obtain a tubular material, wherein the tubular material comprises the synthetic polymer fiber skeleton comprising fiber pores filled with an extracellular matrix of the uniform extracellular matrix solution;
• and
• cross-linking the tubular material with a cross-linking agent, wherein the cross-linking comprises regulating the concentration of the cross-linking agent, regulating cross-linking time to complete the cross-linking, and regulating the pH of the crosslinking reaction in a range of 6 to 8,
• to obtain the composite tubular material;
• wherein the composite tubular material comprises different compounding ratios of the extracellular matrix and the synthetic polymer fiber skeleton obtained by regulating the concentration of the uniform extracellular matrix solution and filling time, wherein the filling time is a time period comprising soaking the synthetic polymer fiber skeleton in the uniform extracellular matrix solution and conducting vacuum pumping until there is no bubble in the uniform extracellular matrix solution.